Trace Element Chromium-D-Phenylalanine Complex: Anti-Inflammatory and Antioxidant Insights from In Vivo and In Silico Studies.

Abstract

The biological significance of trace elements such as chromium extends beyond basic cellular functions, influencing key processes like inflammation and oxidative stress. In this study, we explore the anti-inflammatory and antioxidant potential of a trace element complex, Chromium-D-phenylalanine (Cr(D-Phe)₃), through in vivo and in silico approaches. Anti-inflammatory activity was assessed using a carrageenan-induced paw oedema model in rats, coupled with histopathological and biochemical analyses. The antioxidant effects of Cr(D-Phe)₃ were evaluated by measuring reduced glutathione (GSH), lipid peroxidation (LPO), and tumour necrosis factor-alpha (TNF-α) as a marker of inflammation. Furthermore, molecular docking and dynamics simulations were conducted to elucidate the compound's binding affinity and stability with cyclooxygenase enzymes. Cr(D-Phe)₃ exhibited significant anti-inflammatory activity, with the 40 μg/kg dose achieving 34.40% (p < 0.001) oedema inhibition, comparable to diclofenac sodium (42.40%). Treatment with Cr(D-Phe)₃ restored GSH levels (+ 62.10%, p < 0.001), reduced LPO (24.72%, p < 0.001), and lowered TNF-α (31.73%, p < 0.001) in carrageenan injected rats, demonstrating potent antioxidant and anti-inflammatory effects. Molecular docking revealed strong binding affinities between Cr(D-Phe)₃ and COX enzymes, suggesting its potential mechanism of action in modulating inflammatory pathways. This study highlights the potential of Cr(D-Phe)₃ as a chromium-based trace element complex with anti-inflammatory and antioxidant properties. These findings warrant further preclinical investigations to elucidate its full pharmacological potential and applications in managing inflammatory conditions.