Comparison of two commercial media for corneal organ culture and deswelling: CorneaMax/CorneaJet versus Tissue-C/Carry C

IF 3 3区医学 Q1 OPHTHALMOLOGY

Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.17251

Zhiguo He, Sandrine Ninotta, David Toubeau, Tomy Sagnial, Sylvain Poinard, Oliver Dorado Cortez, Paul Goin, Philippe Gain, Marc Muraine, Gilles Thuret

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Abstract

Aims/Purpose: Corneal storage in organ culture (OC) at 31-34°C is the reference method in Europe. In France, eye banks have traditionally used CorneaMax/CorneaJet media (Eurobio, Les Ulis, France) for several decades. Recently, Tissue-C/Carry-C media (Alchimia-Moria, Antony, France) became available. This study aims at comparing the quality of corneas stored in these two media.

Methods: Eye banks in Saint Etienne and Rouen participated in the study. In Rouen, two experiments were conducted. The first included 18 pairs of corneas, with endothelial cell density (ECD) measured by routine eye bank methods on days -10 and -30 of OC, and 4 days after deswelling. In the second experiment, the protocol for Tissue-C was modified by renewing the medium on day-10. ECD was measured on days -10 and -22 of OC, and 4 days after deswelling. The ECD of 10 pairs of corneas was measured on days -4 and -28 at the Eye Bank of Saint-Etienne. After 2 days of deswelling, the viable ECD was assessed using Hoechst-Calcein-AM staining. Corneal thickness was also measured.

Results: In the first experiment in Rouen, ECD was higher in CorneaMax on day-30 (2202 ± 403 vs 2004 ± 434 cells/mm², p = 0.008) compared to Tissue-C, and higher in CorneaJet after 4 days of deswelling (2088 ± 399 vs 1852 ± 419 cells/mm², p = 0.019) compared to Carry-C. In the second experiment in Rouen, with the medium renewal for Tissue-C and reduced OC duration, the ECD difference disappeared. In Saint Etienne, with medium renewal for Tissue-C during OC, no significant difference in ECD was found during OC. However, the viable ECD of corneas deswelled in CorneaJet was significantly higher than in Carry-C (1647 ± 324 vs 1436 ± 235 cells/mm², p = 0.018).

Conclusions: CorneaMax recommends renewing the medium after the first ECD count, while Tissue-C does not. When following these instructions, CorneaMax showed better ECD results than Tissue-C. However, when the medium for Tissue-C was also renewed during OC, the ECD difference during OC disappeared. The viable ECD indicated that CorneaJet, a deswelling medium was superior to Carry-C. This could be related to the greater thickness of corneas in Tissue-C during OC.

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CorneaMax/CorneaJet与Tissue-C/Carry C两种商用角膜器官培养和肿胀培养基的比较

目的/目的：在31-34℃的器官培养（OC）中保存角膜是欧洲的参考方法。在法国，眼库几十年来一直使用CorneaMax/CorneaJet介质（Eurobio, Les Ulis, France）。最近，Tissue-C/Carry-C培养基（Alchimia-Moria, Antony, France）上市。本研究旨在比较这两种介质中角膜的保存质量。方法：圣艾蒂安和鲁昂的眼库参与研究。在鲁昂进行了两个实验。第一组包括18对角膜，在角膜移植后-10天和-30天以及肿胀后4天，用常规眼库方法测量内皮细胞密度（ECD）。在第二个实验中，通过在第10天更新培养基来修改组织- c的方案。ECD分别于术后第10天和第22天以及肿胀后第4天测量。在圣艾蒂安眼库（Eye Bank of Saint-Etienne）测量10对角膜的ECD，时间分别为第4天和第28天。肿胀2天后，采用Hoechst-Calcein-AM染色评估活ECD。同时测量角膜厚度。结果：在鲁昂的第一个实验中，第30天CorneaMax的ECD高于Tissue-C(2202±403 vs 2004±434 cells/mm2, p = 0.008)，第4天CorneaJet的ECD高于Tissue-C（2088±399 vs 1852±419 cells/mm2, p = 0.019）。在鲁昂的第二个实验中，随着组织c的培养基更新和OC持续时间的缩短，ECD差异消失。在Saint Etienne， OC期间组织- c更新中等，OC期间ECD无显著差异。然而，CorneaJet中角膜肿胀的活ECD显著高于Carry-C（1647±324 vs 1436±235 cells/mm2, p = 0.018）。结论：CorneaMax建议在第一次ECD计数后更换培养基，而Tissue-C则不建议。当遵循这些说明时，CorneaMax的ECD结果优于Tissue-C。然而，当组织- c的培养基也在OC期间更新时，OC期间的ECD差异消失。活性ECD表明，膨胀介质CorneaJet优于Carry-C。这可能与OC期间组织c的角膜厚度较大有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.