Thymus longicaulis subsp. chaubardii Ethanolic Extract Ameliorates Acetic Acid–Induced Rat Ulcerative Colitis Model by Inhibiting Oxidative Stress, Inflammation, and Apoptosis
Dilek Ozbeyli, Ali Sen, Naziye Ozkan Yenal, Ayse Nur Hazar Yavuz, Deniz Mukaddes Turet, Meral Yuksel, Ismail Senkardes, Asli Aykac
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引用次数: 0
Abstract
The search for new drugs to treat ulcerative colitis (UC) is ongoing, with the use of herbal extracts emerging as a current research focus in this field. This study primarily intended to investigate the antioxidant and anti-inflammatory properties of the ethanolic extract of Thymus longicaulis subsp. chaubardii (TLC) in vitro. Secondly, we aimed to perform an acetic acid (AA)–induced rat UC model and assess the effects of TLC extract (200 mg/kg/orally/day, during three days) using ELISA test in terms of IL-1β, TNF-α, IL-17, IL-10, Na+/K+-ATPase, TLR-9, MMP-3 level, caspase-3 and caspase-9, luminol, lucigenin, and SOD. In addition, we accomplished macroscopic and histological evaluations. Our phytochemical investigation revealed TLC contained secondary metabolites including total phenolic, total flavonoid, and total triterpenes in quite concentrated amounts. The extract demonstrated anti-2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity that was 2.3 times more potent than ascorbic acid, anti-2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging activity that was 3.28 times less potent than Trolox, and antilipoxygenase Type V (LOX-5) activity that was 1.31 times more potent than indomethacin in vitro. Due to colitis, increased luminol and lucigenin CL, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, and macroscopic and microscopic scores and decreased IL-10, Na+/K + -ATPase levels in colon ameliorated with TLC and sulfasalazine (as reference drug) treatments. As a result, it can be said that TLC improves UC in rats, mainly on account of its free radical scavenging activity, anti-inflammatory, and antiapoptotic effects.
期刊介绍:
The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet.
Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes:
-Biochemistry of postharvest/postmortem and processing problems
-Enzyme chemistry and technology
-Membrane biology and chemistry
-Cell biology
-Biophysics
-Genetic expression
-Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods
Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following:
-Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease
-The mechanism of the ripening process in fruit
-The biogenesis of flavor precursors in meat
-How biochemical changes in farm-raised fish are affecting processing and edible quality