Effect of Plant Identity in Wheat Mixtures on English Grain Aphid (Sitobion avenae) Control

Abstract

Field experiments have demonstrated that wheat mixtures differ in their ability to regulate aphid populations. To further investigate the effectiveness of wheat mixtures (Triticum aestivum and Triticum turgidum) in controlling aphids, we conducted both laboratory and greenhouse experiments. Specifically, we assessed the associational resistance of two wheat mixtures (Florence-Aurora with Forment, Florence-Aurora with Montcada), and their respective monocultures, in different stages of the aphid host selection process. We analysed aphid acceptance rate, population growth, and load under different wheat treatments. Additionally, we characterised wheat aboveground biomass and nitrogen content as important functional traits for aphid resistant. Aphid acceptance decreased in plants of cv. Forment when exposed to volatiles from undamaged Florence-Aurora plants, whereas the other tested combinations tested had no effect. Aphids performed differently in the two mixtures: Florence-Aurora mixed with Forment significantly reduced aphid population growth and load compared to the monocultures, whereas the combination of Florence-Aurora with Montcada wheat had no effect on aphid performance. The plant–plant interactions also modified the analysed traits. Nitrogen content of Florence-Aurora wheat plants was reduced when mixed with Forment wheat, which may explain the lower aphid load observed in plants of cv. Florence-Aurora when mixed with plants of cv. Forment. However, mixing wheats with similar aboveground biomass resulted in an increase in the average biomass of plants of both cultivars which could have led to a higher aphid population. The data supports the idea of right neighbour, as the benefits of wheat mixtures for aphid control were determined by the identity of the combined plants (or species). Finally, our results suggest that associating wheats with different traits may promote facilitative interactions, which in turn enhances associational resistance, whereas the combination of wheats with similar traits may result in competitive interactions that may hinder aphid control benefits.