Antidiabetic activity of the extracted oil from an Indian indigenous plant, Amberboa ramosa: evidences from in silico, in vitro and enzyme inhibition kinetic studies

Abstract

The emergence of Type-II diabetes mellitus has created a huge epidemiological change, which led humans to think about their lifestyles and health expenditures. Currently, traditional medicines have gained importance in the improvement of lifestyle modifications. Thus, our present studies explore the antidiabetic activity of the extracted oil from the Amberboa ramosa plant, which is a novel approach. The present studies deal with the extraction and identification of the extracted oil and perform computational studies. In addition, DPP-IV enzymatic inhibition and kinetic studies were performed to understand the mode of inhibitions. The results demonstrated that the percentage of valeranone was more (about 15.63%) than the other identified phytocompounds. The docking studies showed binding only with valeranone in the catalytic pocket of the human DPP-IV enzyme. In the enzymatic studies, IC₅₀ was found to be 14.92 μg/ml, and from the secondary plot, the mode of inhibition was mixed. Thus, the extracted oil inhibits the DPP-IV enzyme by binding at the allosteric site.

Graphical abstract