Potential of Industrial Waste to Transfer Microbial Electron in Microbial Fuel Cell Using Dye Reduction Assay

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2025-01-23 DOI:10.1007/s11270-025-07765-x

Ann Maxton, Sam A. Masih

{"title":"Potential of Industrial Waste to Transfer Microbial Electron in Microbial Fuel Cell Using Dye Reduction Assay","authors":"Ann Maxton, Sam A. Masih","doi":"10.1007/s11270-025-07765-x","DOIUrl":null,"url":null,"abstract":"Industries are pillar for nation development, however their development comes with environmental disturbance. Industrial discharge contributes majorly in water pollution however this microbial culture, organic matter rich water serves as an excellent anolyte for Microbial Fuel Cell (MFC). Transfer of electrons is a crucial step in bio electrochemical process catalyzed by microbial community. Methylene Blue reduction method could be an game changer in MFC operations using dye reduction-based electron-transfer activity monitoring (DREAM) assay. Several industrial waste water samples were tested and noticed textile waste water as best suited for DREAM coefficient and electron transportation among other two selected (paper and paint). On screening various microbial dilutions (1x—0.125x) for optimization of DREAM coefficient for maximum power output, the necessity of active microbial populations along with metabolic state was confirmed. Additionally, on screening carbon source suitability (acetate, glucose and sucrose), it was established that maximum DREAM coefficient (0.66 ± 0.03) and power density (91.92 ± 0.81 mW/m2) was achieved with acetate as it is end product of numerous metabolic pathways. Optimized concentration of the best suited carbon source (i.e. acetate) was also analysed and recorded as 25 mmol/L generating maximum power output during MFC operation. Our results established and confirmed that novel DREAM assay as an appropriate, cost effective approach to estimate overall microbial electron exchange as it directly correlates with viable cell count and microbial activity of industrial waste water sample in MFC operation to analyze electrogenic microbial population responsible for bioremediation and green energy production.","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 2","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water, Air, & Soil Pollution","FirstCategoryId":"6","ListUrlMain":"https://link.springer.com/article/10.1007/s11270-025-07765-x","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Industries are pillar for nation development, however their development comes with environmental disturbance. Industrial discharge contributes majorly in water pollution however this microbial culture, organic matter rich water serves as an excellent anolyte for Microbial Fuel Cell (MFC). Transfer of electrons is a crucial step in bio electrochemical process catalyzed by microbial community. Methylene Blue reduction method could be an game changer in MFC operations using dye reduction-based electron-transfer activity monitoring (DREAM) assay. Several industrial waste water samples were tested and noticed textile waste water as best suited for DREAM coefficient and electron transportation among other two selected (paper and paint). On screening various microbial dilutions (1x—0.125x) for optimization of DREAM coefficient for maximum power output, the necessity of active microbial populations along with metabolic state was confirmed. Additionally, on screening carbon source suitability (acetate, glucose and sucrose), it was established that maximum DREAM coefficient (0.66 ± 0.03) and power density (91.92 ± 0.81 mW/m²) was achieved with acetate as it is end product of numerous metabolic pathways. Optimized concentration of the best suited carbon source (i.e. acetate) was also analysed and recorded as 25 mmol/L generating maximum power output during MFC operation. Our results established and confirmed that novel DREAM assay as an appropriate, cost effective approach to estimate overall microbial electron exchange as it directly correlates with viable cell count and microbial activity of industrial waste water sample in MFC operation to analyze electrogenic microbial population responsible for bioremediation and green energy production.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.