Quantification of Aprepitant Via Charge Transfer Complexation Reactions Through Visible Spectrophotometric Methods

Abstract

Two spectrophotometric approaches based on charge transfer complexation processes were developed, validated, and deployed for the detection of aprepitants in pharmaceutical dosages. In method A, the aprepitant reacts para chloranilic acid (p-CA) in acetonitrile, resulting in the formation of a purple-colored product. This product exhibited a maximum absorbance at a wavelength of 524 nm. In technique B, the combination of aprepitant and picric acid (PA) in chloroform results in the generation of chromophores having yellow coloration with a peak absorbance at 415 nm. Every experimental parameter was thoroughly examined and fine-tuned to optimize color development. The validation properties of the recommended spectrophotometric approaches, including linearity, range, precision, accuracy, specificity, robustness, detection, and quantification restrictions, were eff ectively evaluated. Within the concentration range of 10–60 μg/mL, both approaches exhibited a linear relationship with high correlation coefficient values. The suggestions that the processes for quantifying aprepitants in dosage form are straightforward, rapid, precise, accurate, and cost-effective.