Injectable chitosan microspheres resisting inflammatory and oxidative stress for ameliorating intervertebral disc degeneration

IF 6.8 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Science China Materials Pub Date : 2025-01-02 DOI:10.1007/s40843-024-3153-8

Lin Huang (, ), Wantao Wang (, ), Lei Liu (, ), Wenzheng Ma (, ), Jinghao Fan (, ), Dan Zhou (, ), Lei Zhao (, ), Zhaomin Zheng (, ), Hongmei Liu (, ), Decheng Wu (, )

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引用次数: 0

Abstract

The treatment of intervertebral disc (IVD) degeneration remains a significant challenge due to the unique ischemic structure of the IVD, which comprises the scavenging of inflammatory cytokines, alleviation of cellular oxidative stress responses, restoration of nuclei pulposus (NP) cell viability, and recovery of IVD biomechanical function. Herein, we developed an injectable microsphere (CS-MnO₂@PC) by incorporating chitosan microspheres (CS) with manganese dioxide (MnO₂) nanozymes and celecoxib encapsulated in Pluronic F-127 (PC) nanosized micelles, via in situ redox or Schiff base reaction. The hybrid carrier demonstrates robust capabilities in scavenging free radicals, alleviating extracellular oxidative stress, and reducing inflammatory cytokines in NP cells, as evidenced by RT-qPCR and immuno-fluorescence staining assays. In vivo evaluations further indicate that this hybrid carrier helps preserve NP hydration and the lamellar structure of the annulus fibrosus (AF), as confirmed by radiological analysis and histological staining evaluations. These injectable chitosan microspheres, combining nanozymes and nanosized drug micelles, represent a promising therapeutic strategy for degenerative IVD.

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来源期刊

Science China Materials Materials Science-General Materials Science

CiteScore

11.40

自引率

7.40%

发文量

949

期刊介绍： Science China Materials (SCM) is a globally peer-reviewed journal that covers all facets of materials science. It is supervised by the Chinese Academy of Sciences and co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China. The journal is jointly published monthly in both printed and electronic forms by Science China Press and Springer. The aim of SCM is to encourage communication of high-quality, innovative research results at the cutting-edge interface of materials science with chemistry, physics, biology, and engineering. It focuses on breakthroughs from around the world and aims to become a world-leading academic journal for materials science.