Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis

Journal of Leather Science and Engineering Pub Date : 2025-02-02 DOI:10.1186/s42825-024-00183-5

Ming Yang, Xia Li, Bo Wang, Xian Liu, Bo Zhang, Xue-Pin Liao

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Abstract

The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of Saccharomyces cerevisiae against furfural stress. When the furfural concentration was 2 g/L, the residual sugar concentration was reduced from 122.39 to 8.90 g/L, and the final ethanol yield increased from 30.69 to 87.27 g/L in the presence of CP. In addition, the ethanol yield in CP containing media was higher than those in other peptides. Transcriptome analysis showed CP can improve the expression of genes (FBA1, PDC1, PDC6, and ENO1) associated with glycolysis to promote sugar utilization, and enhance ethanol biosynthesis under furfural stress, which were further verified by quantitative real-time PCR. These results indicated that CP is a promising protectant and accelerator for bioethanol biosynthesis.

Graphical abstract

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胶原蛋白肽保护酿酒酵母免受糠醛胁迫，促进生物乙醇合成

高效的生物合成对木质纤维素乙醇工业的可持续发展具有重要意义，但纤维素预处理过程中产生的糠醛胁迫限制了高效生物合成的发展。胶原蛋白肽作为一种丰富的蛋白质资源，可显著提高酿酒酵母对糠醛胁迫的耐受性。当糠醛浓度为2 g/L时，CP的残糖浓度从122.39 g/L降低到8.90 g/L，最终乙醇产率从30.69 g/L提高到87.27 g/L，且含CP培养基的乙醇产率高于其他多肽培养基。转录组分析显示，CP可提高糖酵解相关基因（FBA1、PDC1、PDC6和ENO1）的表达，促进糖的利用，促进糠醛胁迫下乙醇的生物合成，并通过实时荧光定量PCR进一步验证。这些结果表明，CP是一种很有前途的生物乙醇合成保护剂和促进剂。图形抽象

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来源期刊

Journal of Leather Science and Engineering 工程技术-材料科学：综合

CiteScore

12.80

自引率

0.00%

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