Electrochemical detection of non-small cell lung cancer (NSCLC) mir-223 biomarker employing gold/MWCNT nanocomposite–based sandwich platform

IF 2.6 4区化学 Q3 ELECTROCHEMISTRY

Journal of Solid State Electrochemistry Pub Date : 2024-10-03 DOI:10.1007/s10008-024-06094-z

Sangya Bhattacharjee, Melvin George, Bernaurdshaw Neppolian, Jayabrata Das

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引用次数: 0

Abstract

Recently, microRNA-223 (miR-223) has emerged as a new prognostic and diagnostic biomarker for detecting non-small cell lung cancer (NSCLC); thus, sensitive and selective detection of miR-223 is important in the early phase of cancer management. Herein, a simple miR-223 biosensor is developed using a biotin-tagged double-stranded DNA-RNA hybrid structure sandwiched between a recognition probe and a bioconjugate as a signaling unit. The recognition probe (MWCNT/AuNPs/DNA-1//GCE) is fabricated by immobilizing thiol-modified capturer DNA (DNA-1) onto a predesigned multiwall carbon nanotubes/gold nanoparticle–modified glassy carbon electrode (MWCNT/AuNPs//GCE) via Au–S interaction. However, 6-(Ferrocenyl)hexanethiol (Fc-SH) coupled streptavidin/AuNPs bioconjugate (Sv/AuNPs/Fc-SH) can selectively bind to biotinylated dsDNA-RNA hybrid via biotin − streptavidin conjugation and generates electrooxidation signal directly under applied potential. The proposed sensor demonstrates linear dynamic response as a function of log concentration of miR-223 (log C_miR-223) ranging from 1 pM to 10 nM with a relatively low detection limit of 0.73 pM (3σ/sensitivity, n = 3) and is capable of discriminating miR-223 from its homologous sequences, hence can be considered for the diagnosis of clinical samples.

Graphical Abstract

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利用金/MWCNT纳米复合材料夹层平台电化学检测非小细胞肺癌（NSCLC） mir-223生物标志物

最近，microRNA-223 （miR-223）已成为检测非小细胞肺癌（NSCLC）的一种新的预后和诊断生物标志物；因此，敏感和选择性地检测miR-223在癌症治疗的早期阶段是重要的。本文采用生物素标记的双链DNA-RNA杂交结构，夹在识别探针和生物偶联物之间作为信号单元，开发了一种简单的miR-223生物传感器。该识别探针（MWCNT/AuNPs/DNA-1//GCE）是通过Au-S相互作用将巯基修饰的捕获体DNA （DNA-1）固定在预先设计的多壁碳纳米管/金纳米颗粒修饰的玻璃碳电极（MWCNT/AuNPs//GCE）上制备的。然而，6-（二铁基）己硫醇（Fc-SH）偶联链亲和素/AuNPs生物偶联物（Sv/AuNPs/Fc-SH）可以通过生物素-链亲和素偶联选择性结合生物素化的dsDNA-RNA杂合体，并在施加电位下直接产生电氧化信号。该传感器的线性动态响应是miR-223对数浓度（log CmiR-223）在1 pM至10 nM范围内的函数，相对较低的检测限为0.73 pM (3σ/灵敏度，n = 3)，能够将miR-223与其同源序列区分出来，因此可以考虑用于临床样品的诊断。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Solid State Electrochemistry 工程技术-电化学

CiteScore

4.80

自引率

4.00%

发文量

227

审稿时长

4.1 months

期刊介绍： The Journal of Solid State Electrochemistry is devoted to all aspects of solid-state chemistry and solid-state physics in electrochemistry. The Journal of Solid State Electrochemistry publishes papers on all aspects of electrochemistry of solid compounds, including experimental and theoretical, basic and applied work. It equally publishes papers on the thermodynamics and kinetics of electrochemical reactions if at least one actively participating phase is solid. Also of interest are articles on the transport of ions and electrons in solids whenever these processes are relevant to electrochemical reactions and on the use of solid-state electrochemical reactions in the analysis of solids and their surfaces. The journal covers solid-state electrochemistry and focusses on the following fields: mechanisms of solid-state electrochemical reactions, semiconductor electrochemistry, electrochemical batteries, accumulators and fuel cells, electrochemical mineral leaching, galvanic metal plating, electrochemical potential memory devices, solid-state electrochemical sensors, ion and electron transport in solid materials and polymers, electrocatalysis, photoelectrochemistry, corrosion of solid materials, solid-state electroanalysis, electrochemical machining of materials, electrochromism and electrochromic devices, new electrochemical solid-state synthesis. The Journal of Solid State Electrochemistry makes the professional in research and industry aware of this swift progress and its importance for future developments and success in the above-mentioned fields.