Electrochemical detection of non-small cell lung cancer (NSCLC) mir-223 biomarker employing gold/MWCNT nanocomposite–based sandwich platform

IF 2.6 4区化学 Q3 ELECTROCHEMISTRY

Journal of Solid State Electrochemistry Pub Date : 2024-10-03 DOI:10.1007/s10008-024-06094-z

Sangya Bhattacharjee, Melvin George, Bernaurdshaw Neppolian, Jayabrata Das

{"title":"Electrochemical detection of non-small cell lung cancer (NSCLC) mir-223 biomarker employing gold/MWCNT nanocomposite–based sandwich platform","authors":"Sangya Bhattacharjee, Melvin George, Bernaurdshaw Neppolian, Jayabrata Das","doi":"10.1007/s10008-024-06094-z","DOIUrl":null,"url":null,"abstract":"<div><p>Recently, microRNA-223 (miR-223) has emerged as a new prognostic and diagnostic biomarker for detecting non-small cell lung cancer (NSCLC); thus, sensitive and selective detection of miR-223 is important in the early phase of cancer management. Herein, a simple miR-223 biosensor is developed using a biotin-tagged double-stranded DNA-RNA hybrid structure sandwiched between a recognition probe and a bioconjugate as a signaling unit. The recognition probe (MWCNT/AuNPs/DNA-1//GCE) is fabricated by immobilizing thiol-modified capturer DNA (DNA-1) onto a predesigned multiwall carbon nanotubes/gold nanoparticle–modified glassy carbon electrode (MWCNT/AuNPs//GCE) via Au–S interaction. However, 6-(Ferrocenyl)hexanethiol (Fc-SH) coupled streptavidin/AuNPs bioconjugate (Sv/AuNPs/Fc-SH) can selectively bind to biotinylated dsDNA-RNA hybrid via biotin − streptavidin conjugation and generates electrooxidation signal directly under applied potential. The proposed sensor demonstrates linear dynamic response as a function of log concentration of miR-223 (log C<sub>miR-223</sub>) ranging from 1 pM to 10 nM with a relatively low detection limit of 0.73 pM (3σ/sensitivity, <i>n</i> = 3) and is capable of discriminating miR-223 from its homologous sequences, hence can be considered for the diagnosis of clinical samples.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":665,"journal":{"name":"Journal of Solid State Electrochemistry","volume":"29 2","pages":"669 - 680"},"PeriodicalIF":2.6000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10008-024-06094-z.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Solid State Electrochemistry","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10008-024-06094-z","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ELECTROCHEMISTRY","Score":null,"Total":0}

引用次数: 0

Abstract

Recently, microRNA-223 (miR-223) has emerged as a new prognostic and diagnostic biomarker for detecting non-small cell lung cancer (NSCLC); thus, sensitive and selective detection of miR-223 is important in the early phase of cancer management. Herein, a simple miR-223 biosensor is developed using a biotin-tagged double-stranded DNA-RNA hybrid structure sandwiched between a recognition probe and a bioconjugate as a signaling unit. The recognition probe (MWCNT/AuNPs/DNA-1//GCE) is fabricated by immobilizing thiol-modified capturer DNA (DNA-1) onto a predesigned multiwall carbon nanotubes/gold nanoparticle–modified glassy carbon electrode (MWCNT/AuNPs//GCE) via Au–S interaction. However, 6-(Ferrocenyl)hexanethiol (Fc-SH) coupled streptavidin/AuNPs bioconjugate (Sv/AuNPs/Fc-SH) can selectively bind to biotinylated dsDNA-RNA hybrid via biotin − streptavidin conjugation and generates electrooxidation signal directly under applied potential. The proposed sensor demonstrates linear dynamic response as a function of log concentration of miR-223 (log C_miR-223) ranging from 1 pM to 10 nM with a relatively low detection limit of 0.73 pM (3σ/sensitivity, n = 3) and is capable of discriminating miR-223 from its homologous sequences, hence can be considered for the diagnosis of clinical samples.

Graphical Abstract

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Solid State Electrochemistry 工程技术-电化学

CiteScore

4.80

自引率

4.00%

发文量

227

审稿时长

4.1 months

期刊介绍： The Journal of Solid State Electrochemistry is devoted to all aspects of solid-state chemistry and solid-state physics in electrochemistry. The Journal of Solid State Electrochemistry publishes papers on all aspects of electrochemistry of solid compounds, including experimental and theoretical, basic and applied work. It equally publishes papers on the thermodynamics and kinetics of electrochemical reactions if at least one actively participating phase is solid. Also of interest are articles on the transport of ions and electrons in solids whenever these processes are relevant to electrochemical reactions and on the use of solid-state electrochemical reactions in the analysis of solids and their surfaces. The journal covers solid-state electrochemistry and focusses on the following fields: mechanisms of solid-state electrochemical reactions, semiconductor electrochemistry, electrochemical batteries, accumulators and fuel cells, electrochemical mineral leaching, galvanic metal plating, electrochemical potential memory devices, solid-state electrochemical sensors, ion and electron transport in solid materials and polymers, electrocatalysis, photoelectrochemistry, corrosion of solid materials, solid-state electroanalysis, electrochemical machining of materials, electrochromism and electrochromic devices, new electrochemical solid-state synthesis. The Journal of Solid State Electrochemistry makes the professional in research and industry aware of this swift progress and its importance for future developments and success in the above-mentioned fields.