Brucine-Loaded Nickel oxide/Sodium alginate/Polyethylene glycol Nanocomposites Mitigates Inflammatory Responses and Oxidative stress in imiquimod-induced Psoriasis-Like Skin Inflammation in Mice

Abstract

Background

Psoriasis is a chronic inflammatory disease with an unpredictable pathophysiology and no proper cure. Nanomedicine is the use of nanotechnology in the medical field to diagnose, treat, and prevent diseases.

Objective

The objective of this work is to prepare and characterize the NiO/PEG/SA-Brucine NCs and evaluate their therapeutic effects against (imiquimod) IMQ-induced psoriasis in mice.

Methodology

The synthesized NiO/PEG/SA-Brucine NCs were characterized using several methods. The effect of NiO/PEG/SA-Brucine NCs on the viability and inflammatory cytokines of LPS-induced RAW 264.7 cells was assessed. The psoriasis was induced in the experimental mice using IMQ and topically treated with 10 and 20 mg/kg of NiO/PEG/SA-Brucine NCs. The levels of inflammatory markers, pro-inflammatory cytokines, and oxidative stress markers in the experimental mice were assessed using the kits. The histomorphometrical analysis was conducted on the ear and skin tissue samples of the experimental mice.

Results

The treatment with the synthesized NiO/PEG/SA-Brucine NCs effectively increased cell viability and reduced inflammatory markers in the LPS-induced RAW 264.7 cells. In the psoriasis-induced mice, the NiO/PEG/SA-Brucine NCs treatment successfully reduced inflammatory cell counts and improved histomorphometrical changes. The NiO/PEG/SA-Brucine NCs effectively decreased the inflammatory markers in the psoriasis mice. The NCs treatment also increased the antioxidant levels and mitigated the skin histological changes in the psoriasis mice.

Conclusion

The findings of this study revealed that NiO/PEG/SA-Brucine NCs successfully mitigated the IMQ-induced psoriatic condition in mice. Consequently, it was evident that NiO/PEG/SA-Brucine NCs may be a beneficial option for treating psoriasis.