Genome-wide identification, evolution, diversity and expression of Caspase gene family in Haliotis discus hannai

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2024-11-22 DOI:10.1016/j.aquaculture.2024.741900

Yuping Chen , Wangying Wu , Hui Ge , Zhen Zhao , Hanling Gan , Nan Chen , Jianpeng Huang , Chao Fan , Mingyi Cai , Yilei Wang , Ziping Zhang

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引用次数: 0

Abstract

Apoptosis is crucial in the immune defense mechanisms of mollusks. In this study, 25 Caspase genes were identified from Haliotis discus hannai’ genome. Through structural and evolutionary relationship analysis, highly conserved data were observed between H. discus hannai and other mollusks in the Caspase. Notably, the first discovery of Caspase-9 in abalones in this study. Collinearity analysis detected numerous tandem duplication events within the Caspase genes, implicating these duplications as the principal evolutionary driver of the Caspase family in H. discus hannai. Sequence diversity analysis found 216 missense mutation sites in Caspase genes of H. discus hannai. Alternative splicing analysis revealed multiple splicing sites and events in Caspase of H. discus hannai. Additionally, differential alternative splicing analysis showed the significant induction of Caspase-2, 3, and 8 variants following Vibrio infection. Transcriptome quantitative and qRT-PCR demonstrated that most Caspase genes in H. discus hannai respond to heat and Vibrio inoculation. Our findings offer novel perspectives on Caspase function and immune modulation mechanisms in H. discus hannai.

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.