Nanocomposite hydrogel-based bioinks composed of a fucose-rich polysaccharide and nanocellulose fibers for 3D-bioprinting applications

Q1 Computer Science

Bioprinting Pub Date : 2025-02-01 DOI:10.1016/j.bprint.2024.e00382

Nicole S. Lameirinhas , João P.F. Carvalho , Maria C. Teixeira , Jorge L. Luís , Asiyah Esmail , Ricardo J.B. Pinto , Helena Oliveira , Filomena Freitas , José M. Oliveira , Carla Vilela , Armando J.D. Silvestre , Carmen S.R. Freire

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引用次数: 0

Abstract

Hydrogels are the most common type of bioinks, yet, finding adequate biomaterials to develop suitable bioinks for 3D bioprinting remains challenging. Herein, innovative hydrogel bioinks were developed by combining nanofibrillated cellulose (NFC) with a fucose-rich polysaccharide, FucoPol (FP), still unexplored for 3D bioprinting. NFC/FP bioinks with different mass proportions, namely 1:1, 2:1, 3:1 and 4:1, were prepared and denominated as NFC1FP, NFC2FP, NFC3FP and NFC4FP. A formulation without NFC was also prepared for comparison purposes (NFC0FP). The rheological properties of the bioinks were enhanced by the addition of NFC, as evidenced by the increase in shear viscosity from 1.39 ± 0.03 Pa s (NFC0FP) to 2933.7 ± 137.9 Pa s (ink NFC4FP) and by the 3D printing of complex structures with high shape fidelity (Pr ≈ 0.9). The stability and mechanical properties of the crosslinked hydrogels were also improved, with Young’s modulus increasing from 0.12 ± 0.04 MPa (NFC0FP) to 2.45 ± 0.06 MPa (NFC4FP). The successful 3D bioprinting of both A375 (melanoma) and HaCaT (keratinocyte) cell-laden bioinks translated into elevated cell viabilities (above 88 %) up to 21 days post-bioprinting. These results highlight the potential and versatility of NFC/FP bioinks for the bioprinting of 3D skin tissue analogues for biomedical applications.

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.