A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans

IF 4.9 Q1 MICROBIOLOGY
Fruzsina Kovács , Ágnes Jakab , Noémi Balla , Zoltán Tóth , Dávid Balázsi , Lajos Forgács , Andrea Harmath , Aliz Bozó , Ágota Ragyák , László Majoros , Renátó Kovács
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引用次数: 0

Abstract

Candida auris occupies similar niches in various infections as Pseudomonas aeruginosa; however, the details of their interspecies communication remain largely unknown. To gain deeper insights into this relationship, phenotypic and transcriptomic analyses were conducted in the presence of the primary P. aeruginosa quorum-sensing molecule, N-(3-oxododecanoyl)-l-homoserine lactone (HSL), against C. auris, with the results compared to those of C. albicans. Our findings indicate that HSL-induced effects are not specific to C. albicans; additionally, several characteristics are present in C. auris but not in C. albicans following HSL exposure. Significant HSL-induced reduction was observed in growth and adhesion of C. auris cells in time -and concentration-dependent way (p < 0.01-0.001). Moreover, HSL reduced intracellular iron and zinc levels (p < 0.05-0.001); furthermore, it modulated C. auris metabolism toward beta-oxidation, which may be associated with the observed reduction in in vivo virulence at lower HSL concentrations compared with C. albicans. RNA-sequencing transcriptome analysis of C. auris revealed 67 and 306 upregulated genes, as well as 111 and 168 downregulated genes, in response to 100 and 200-μM HSL, respectively. We identified 45 overlapping upregulated and 25 overlapping downregulated genes between the two HSL concentrations. Similar to other Candida-derived C12 compounds (e.g., farnesol), HSL reduces several C. auris survival strategies, which may significantly influence the nature of P. aeruginosa–C. auris co-habitation. In conclusion, the obtained findings on C. auris do not provide clear evidence that HSL mediated effects have any favourable consequences in terms of P. aeruginosa-C. auris co-colonisation and/or co-infections.
群体感应分子3-氧- c12 -同丝氨酸内酯对耳念珠菌和白色念珠菌影响的综合分析
金黄色念珠菌在各种感染中与铜绿假单胞菌占据相似的生态位;然而,它们的物种间交流的细节在很大程度上仍然未知。为了更深入地了解这种关系,在铜绿假单胞菌主要群体感应分子N-(3-氧十二烷基)-l-高丝氨酸内酯(HSL)存在的情况下,对金黄色葡萄球菌进行了表型和转录组学分析,并将结果与白色念珠菌的结果进行了比较。我们的研究结果表明hsl诱导的效应并不局限于白色念珠菌;此外,暴露于HSL后,耳念珠菌有几个特征,而白色念珠菌没有。hsl诱导的金黄色葡萄球菌细胞生长和粘附均呈时间和浓度依赖性(p <;0.01 - -0.001)。此外,HSL降低细胞内铁和锌水平(p <;0.05 - -0.001);此外,它调节了金黄色葡萄球菌向β -氧化的代谢,这可能与观察到的较低HSL浓度下与白色念珠菌相比体内毒力降低有关。rna测序转录组分析显示,C. auris在100 μ m和200 μ m HSL环境下分别表达了67个和306个上调基因,111个和168个下调基因。我们发现在两种HSL浓度之间有45个重叠的上调基因和25个重叠的下调基因。与其他假丝酵母衍生的C12化合物(如法尼醇)类似,HSL降低了几种金黄色葡萄球菌的生存策略,这可能显著影响铜绿假单胞菌c的性质。奥瑞丝同居。总之,获得的关于金黄色葡萄球菌的研究结果并没有提供明确的证据表明HSL介导的效应对铜绿假单胞菌c有任何有利的影响。耳共定植和/或共感染。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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