Molecular cloning and functional characterizations of transient receptor potential A1 (TRPA1) in Aedes albopictus

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2025-01-09 DOI:10.1016/j.pestbp.2025.106295

Haiyan Lv , Guanlong Wang , Xinheng Wu , Dingxin Jiang

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Abstract

Aedes albopictus is a potent vector of major arboviruses that cause serious health burdens and economic losses in worldwide. To facilitate the exploration of potential mosquito repellents, the Ae. albopictus TRPA1 (AalTRPA1) gene was cloned and investigated its molecular characteristics by bioinformatics techniques and the function using electrophysiological and RNAi techniques. In vitro expression and electrophysiological analysis of Xenopus oocytes showed that high temperature and allyl isothiocyanate (AITC) could activate the AalTRPA1 channel. After interfering with this gene, Ae. albopictus was insensitive to temperature changes, was more easily hurt and shot down by noxious heat; it was also insensitive to catnip, and the effect of repellent was poor. We firstly identified the molecular characterization of AalTRPA1 subfamily genes and their key role in temperature and irritant perception in Ae. albopictus, and then the AalTRPA1 has the potential to develop a new mosquito repellent target against Ae. albopictus.

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.