Peptide neurotoxins affecting insect voltage-gated calcium channels and possessing insecticidal toxicity: Two ω-Atypitoxins from Calommata signata

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yang-yang Yan, Kan Wang, Jing-ting Wang, Qian-qian Han, Zhen Zhang, Na Yu, Ze-Wen Liu
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Abstract

Spider peptide toxins, as potent insecticides, distinguish them from current insecticides, and thus expand the range of viable molecular targets of insecticides in insect pests. This study reports the isolation of two neurotoxic peptides, ω-Atyptoxin-Cs2a (Cs2a) and ω-Atyptoxin-Cs2b (Cs2b), from the venom gland of the burrowing spider Calommata signata. These peptides exhibit strong insecticidal activity against two insect pests with agricultural importance, Nilaparvata lugens and Spodoptera frugiperda. Both toxins demonstrated rapid and significant lethality, with LD50 values of 1.083 ± 0.120 nmol/g and 0.949 ± 0.079 nmol/g for N. lugens and 1.035 ± 0.114 nmol/g and 0.998 ± 0.081 nmol/g for S. frugiperda within 24 h. Using the whole-cell patch-clamp electrophysiology, Cs2a and Cs2b were identified as inhibitors of high-voltage-activated calcium channels in cockroach DUM neurons, with IC50 values of 0.504 ± 0.078 μM and 0.411 ± 0.053 μM, respectively. These findings show that Cs2a and Cs2b are effective calcium channel blockers with potential to develop bioinsecticides, offering selective toxicity toward insect pests.

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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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