Combined transcriptome and metabolome analysis provides insight into the ERF073 – Malic acid network in pakchoi under submergence stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2024-12-12 DOI:10.1016/j.stress.2024.100708

Dan-dan Xi , Lu Gao , Li-ao Ge , Hong-fang Zhu , Li-ming Miao , Ding-yu Zhang , Chang-wei Zhang , Ying Li , Yan-xiao Dong , Xi-lin Hou , Yu-ying Zhu , Zhao-hui Zhang , Xiao-feng Li

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引用次数: 0

Abstract

Pakchoi is sensitive to oxygen deficiency caused by submergence; however, the molecular mechanisms underlying its response to these conditions remains unclear. To determine these mechanisms, two pakchoi cultivars, Heiyoudong (HYD) and Suzhouqing (SZQ), were subjected to submergence for 1 h and 5 h, respectively. Following this treatment, the plants were transferred back to the control conditions for recovery. HYD exhibited higher survival rate than SZQ after recovery. Leaves from 1 h stress, 5 h stress, and subsequent 5-h recovery phases were harvested for metabolome and transcriptome analyses. Data analysis revealed that 206 differentially accumulated metabolites (DAMs) were identified between SZQ and HYD after 1 h stress (S1 vs H1) and 330 DAMs were identified between SZQ and HYD after 5 h stress (S5 vs H5) at stress stage. During recovery stage, 124 and 310 DAMs were found in S1 vs H1 and S5 vs H5, respectively. Additionally, 553 commonly differentially expressed genes (DEGs) were found both in S1 vs H1 and S5 vs H5 at the stress stage, while 458 DEGs were commonly found in the two comparison groups at the recovery stage. Among these DEGs, ERF073 was down-regulated, as further confirmed by qRT-PCR. KEGG pathway analysis revealed that DAMs and DEGs were mainly enriched in metabolic pathways. Taken together, these findings indicate that ERF073 may regulate malic acid accumulation in pakchoi, increasing its resistance to submergence.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.