PsDUF6A from Populus simonii enhances drought tolerance in transgenic Arabidopsis and poplar by increasing ROS scavenging

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2024-12-13 DOI:10.1016/j.stress.2024.100706

Yanfei Yang , Jinna Zhao , Xingrong Ren , Xueqi Bai , Tao Li , Jianbo Li

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引用次数: 0

Abstract

Domain of unknown function (DUF) proteins play roles in a range of plant biological processes, including growth and development, and adaptation to abiotic stresses. However, their function was largely unknown in woody plants. Populus simonii is a notable native tree species in northern China and is highly tolerance to drought stress. In this study, PsDUF6A was isolated and functionally characterized from P. simonii. This gene was highly expressed in mature leaves and its expression was induced under drought condition. Transgenic Arabidopsis and 84 K poplar lines overexpressing PsDUF6A were constructed to investigate the function of PsDUF6A in drought tolerance. Under drought conditions, the survival rate and relative water content were higher in PsDUF6A-overexpressing Arabidopsis than in wild-type Arabidopsis, whereas the opposite trend was observed for relative electrical conductivity, indicative of increased drought tolerance. Compared with 84 K poplar, transgenic poplar had a higher photosynthetic activity, lower water loss rate, and higher root biomass. Moreover, PsDUF6A-overexpressing increased antioxidant enzyme activities and the reactive oxygen species scavenging. In addition, the yeast one-hybrid assay indicated that PsC2H213, PsC2H214, PsC2H215, PsC2H217, and PsC2H218 can directly bind to PsDUF6A promoter. These results indicated that PsDUF6A enhances drought tolerance by maintaining ROS homeostasis, and its expression might regulate by C2H2-type ZFPs. These findings revealed the positive contributions of PsDUF6A to drought tolerance and provided insights into the underlying regulatory network of P. simonii response to drought stress.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.