Performance and mechanisms of heterotrophic nitrification aerobic denitrifying bacteria in utilizing photogenerated electrons from magnetite for efficient denitrification

IF 6.3 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-02-01 DOI:10.1016/j.jwpe.2025.106942

Guo Liu , Dian Liu , Mengyao Hu , Xi Ren , Yueyu Ran , Tianlie Luo , Willie J.G.M. Peijnenburg

{"title":"Performance and mechanisms of heterotrophic nitrification aerobic denitrifying bacteria in utilizing photogenerated electrons from magnetite for efficient denitrification","authors":"Guo Liu , Dian Liu , Mengyao Hu , Xi Ren , Yueyu Ran , Tianlie Luo , Willie J.G.M. Peijnenburg","doi":"10.1016/j.jwpe.2025.106942","DOIUrl":null,"url":null,"abstract":"<div><div>Heterotrophic nitrification-aerobic denitrification bacteria (HN-AD) have been demonstrated to possess denitrification potential. The limited effectiveness of HN-AD in remediating nitrogen-polluted surface waters is attributed to its low C/N ratio. In this study, a magnetite photogenerated electrons coupled HN-AD bacteria system was constructed and its nitrogen removal mechanisms were revealed. The results demonstrated the electrons photo-generated by magnetite can effectively stimulate the growth of the HN-AD <em>(Delftia sp.,</em> Y19).The coupled system of the ammonium and nitrate reached removal rates of 80.1 % and 71.3 %, which were 4 times higher than the strain Y19 alone (20.3 %, 15.2 %). Compared with dark conditions, the activity of enzymes (AMO, HAO, NAR and NIR) related to nitrogen removal in Y19 was increased by 4.81, 4.75, 6.45 and 4.78 times under sunlight irradiation, respectively. This suggests that the electrons photo-generated from magnetite can enhance the metabolic activities of the Y19 strain. Furthermore, the concentration of ferric ions dissolved from magnetite has been detected as equaling 0.13 mg/L, which plays a crucial role in the reduction of nitrate. The denitrification mechanisms of the coupled system can be incorporated: heterotrophic nitrification and aerobic denitrification by strain Y19, photogenerated electrons reduction of magnetite, the reduction of ferric ions, and the adsorption of magnetite. After 9 days of running the simulator, the magnetite-Y19 coupled system achieved removal rates of 100 % for nitrate and chemical oxygen demand, and 36 % for ammonium. This study offers novel insights into the utilization of photogenerated electrons by microorganisms for remediating the low C/N ratio wastewater.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"70 ","pages":"Article 106942"},"PeriodicalIF":6.3000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425000145","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Heterotrophic nitrification-aerobic denitrification bacteria (HN-AD) have been demonstrated to possess denitrification potential. The limited effectiveness of HN-AD in remediating nitrogen-polluted surface waters is attributed to its low C/N ratio. In this study, a magnetite photogenerated electrons coupled HN-AD bacteria system was constructed and its nitrogen removal mechanisms were revealed. The results demonstrated the electrons photo-generated by magnetite can effectively stimulate the growth of the HN-AD (Delftia sp., Y19).The coupled system of the ammonium and nitrate reached removal rates of 80.1 % and 71.3 %, which were 4 times higher than the strain Y19 alone (20.3 %, 15.2 %). Compared with dark conditions, the activity of enzymes (AMO, HAO, NAR and NIR) related to nitrogen removal in Y19 was increased by 4.81, 4.75, 6.45 and 4.78 times under sunlight irradiation, respectively. This suggests that the electrons photo-generated from magnetite can enhance the metabolic activities of the Y19 strain. Furthermore, the concentration of ferric ions dissolved from magnetite has been detected as equaling 0.13 mg/L, which plays a crucial role in the reduction of nitrate. The denitrification mechanisms of the coupled system can be incorporated: heterotrophic nitrification and aerobic denitrification by strain Y19, photogenerated electrons reduction of magnetite, the reduction of ferric ions, and the adsorption of magnetite. After 9 days of running the simulator, the magnetite-Y19 coupled system achieved removal rates of 100 % for nitrate and chemical oxygen demand, and 36 % for ammonium. This study offers novel insights into the utilization of photogenerated electrons by microorganisms for remediating the low C/N ratio wastewater.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies