Exploring mechanisms of resistance to fludioxonil in Colletotrichum fructicola

Abstract

Colletotrichum fructicola is one of the most important species causing peach anthracnose around the world, including China. Fludioxonil has been effectively applied to control anthracnose disease as well as several important fungal diseases such as gray mold, leaf blight, early blight, corn stem rot, peanut root rot, rice evil seedling disease, and other diseases transmitted through seeds or soil. In this study, 39C. fructicola isolates were collected from different locations in Guizhou Province and Guangdong Province. A sensitive isolate of C. fructicola, previously thought to be naturally resistant to fludioxonil, was unexpectedly detected. No cross-resistance was found for fludioxonil with procymidone, prochloraz, and pyraclostrobin. Significant differences were observed between sensitive and resistant isolates in terms of mycelial growth rate and osmotic sensitivity experiments (4 %, 6 %, and 8 % NaCl), but no significant difference was found for sporulation. A novel mutation I880V was detected in the Os1 protein from one sensitive isolate. Molecular docking was used to explain the reversal of inherent resistance to sensitivity of C. fructicola to fludioxonil. Results showed that the wild type Os1 protein was docked against fludioxonil with a binding energy of −6.8 kj/mol, while it increased to −6.6 kj/mol between the mutated protein and fludioxonil. At the same time, different interactions were observed between wild type and mutated proteins with fludioxonil. These results suggest that the I880V mutation in the Os1 protein changed the conformation of the binding pocket, potentially leading to the reversal from resistance to sensitivity to fludioxonil. These findings are remarkable in demonstrating the fludioxonil resistance mechanism, and further studies such as genetic transformation and a range of molecular investigations are necessary to validate resistance mechanisms, elucidate the molecular pathways involved, and develop effective disease management strategies.