Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-12-30 DOI:10.1016/j.pestbp.2024.106281

Zhichao Luo , Haoting Chen , Daojie Guan , Chen Hang , Zhichao Wang , Huichen Ge , Kun Qian , Jianjun Wang

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引用次数: 0

Abstract

It is well known that the transcription factors aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) play important roles in the detoxification of xenobiotic compounds including insecticides. In this study, TcAhR and TcARNT were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcAhR and TcARNT were highly expressed in brain and epidermis of female adults and upregulated after exposure of the adults to deltamethrin. Knockdown of TcAhR and TcARNT increased the susceptibility of T. castaneum to deltamethrin and decreased the enzymatic activity of Glutathione S-transferase (GST). Consistently, knockdown of TcAhR and TcARNT led to the downregulation of TcGSTs6 and suppressed the induction of TcGSTs6 by deltamethrin treatment. Increased susceptibility of T. castaneum to deltamethrin was also observed after knockdown of TcGSTs6. Further dual-luciferase reporter assay revealed that co-overexpression of TcAhR and TcARNT in Sf9 cells significantly enhanced the promoter activity of TcGSTs6. These results provided insights into the function of insect AhR and ARNT as well as the regulatory mechanisms of insect GSTs.

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.