Novel reporter based on Aggregation-induced emission Luminogens for lateral flow immunoassay: A mini review

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-02-01 DOI:10.1016/j.trac.2024.118098

Gan Zhang , Sha Yu , Juan Peng , Yonghua Xiong , Liming Hu , Weihua Lai

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Abstract

Fluorescent lateral flow immunoassay (LFIA), known for their robust signal-readout capabilities, is approximately 1–2 orders of magnitude more sensitive than traditional LFIA. However, most fluorescent materials suffer from aggregation-caused quenching (ACQ) at high concentration, which restricted the performance of these fluorescent reporters. Aggregation-induced emission luminogens (AIEgens) against ACQ have demonstrated exceptional optical performance, garnering significant attentions in immunoassay. Here, we have conducted an extensive review of AIEgens-based LFIA in three main aspects: (1) AIE-based reporters including single AIEgens, non-dyes typed AIE materials, and AIEgens-loading typed fluorescent microspheres; (2) coupling strategies including electrostatic adsorption, EDC method, oriented antibody coupling, and surface modifications; (3) potential typed AIE-LFIAs including molecular engineering, AIEgens doping, and novel confinement strategy for AIEgens. Especially, the future challenges and perspectives of AIEgens-based LFIA are also discussed in detail, aiming to provide guidance for the development of high-performance AIEgens-based LFIA in wide application field.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.