A novel dual-function biomimetic approach for high throughput organic dye biodegradation and hydrogen peroxide sensing using a nanosized artificial peroxidase with ultra-improved substrate affinity and superb catalytic efficiency

Abstract

A high throughput novel dual-function biomimetic artificial enzyme-based approach was developed for dye biodegradation and hydrogen peroxide quantification using a nanosized artificial peroxidase with superb catalytic efficiency and ultra-improved substrate affinity compared to the native enzyme. The artificial peroxidase was chemically, kinetically, and biochemically characterized. A high specific activity of 0.0503 UI mg⁻¹, an optimal-pH of 4.0, an optimal-temperature of 35 ℃, and a high storage stability for at-least 30 days was determined for this nanozyme. It retained 88.2 % and 90 % of its activity at 60 ℃ (200 min) and 5 cycles, respectively, revealing its high thermal and cycling stability. Its catalytic efficiency and substrate affinity were 3.2-fold and 7.1-order higher than the native peroxidase, respectively. The mechanistic insights were provided for the activity of nanozyme. A dye biodegrading protocol with a yield of 99.1 % (75.2 % after 10 reuses) within 30.0 min was developed and applied for dye degradation from real waters and textile wastewater. An analytical highly sensitive, reproducible, and selective H₂O₂ biosensor was also designed, providing a linear quantification range over 1.0–180.0 µM, a detection limit of 0.23 µM, and accurate results for milk analysis. This method showed very better figures of merits than reported ones.