Cadmium exposure induced spleen inflammation by activating the MAPK/NF-κB/ NLRP3 signaling pathway and the intervention effect of astilbin

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-01-31 DOI:10.1016/j.vetimm.2025.110889

Tiezhu Chen , Tianyu Han , Yusong Miao , Liangchun Yan , Zhijun Liu , Han Dong , Tingting Cheng , Yiding Liu , Yu Yang , Shanshan Fei , Guoqiang Cheng , Guangliang Shi

{"title":"Cadmium exposure induced spleen inflammation by activating the MAPK/NF-κB/ NLRP3 signaling pathway and the intervention effect of astilbin","authors":"Tiezhu Chen , Tianyu Han , Yusong Miao , Liangchun Yan , Zhijun Liu , Han Dong , Tingting Cheng , Yiding Liu , Yu Yang , Shanshan Fei , Guoqiang Cheng , Guangliang Shi","doi":"10.1016/j.vetimm.2025.110889","DOIUrl":null,"url":null,"abstract":"<div><div>Cadmium (Cd) is an environmental pollutant with strong immunotoxicity effects. Despite this, the mechanisms by which Cd causes spleen damage in chickens are not well understood. Astilbin (ASB) is a dihydroflavone glycoside with anti-inflammatory and anti-oxidation properties. In the present study, a chicken spleen injury model induced by cadmium exposure (90d) was established to explore the specific mechanisms of Cd-induced spleen injury. If and how ASB ameliorates the damage was also explored. A total of 60 chickens were randomly divided into four groups: Con, Cd, ASB, and Cd+ASB groups. The pathological changes in the spleen were observed by H&E staining. Cd-induced oxidative stress, inflammation, and the involvement of the MAPK/NF-κB/NLRP3 in ameliorating spleen damage were also analyzed by Western blotting, qRT-PCR, and immunohistochemistry. Our results showed that Cd exposure for 90 days damaged the spleen, which mainly manifested as eosinophil infiltration, an increase in MDA content, a decrease in the CAT, GSH, SOD, and T-AOC, and activation of MAPK/NF-κB/NLRP3 signaling pathway. The overall outcome of these events was the induction of oxidative stress and inflammation in the spleen of the chickens. Interestingly, ASB treatment ameliorated Cd-induced damages. In conclusion, the present study revealed the specific mechanism of Cd-induced spleen damage using a chicken model. But ASB ameliorates Cd-induced cadmium poisoning.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"281 ","pages":"Article 110889"},"PeriodicalIF":1.4000,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary immunology and immunopathology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165242725000091","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cadmium (Cd) is an environmental pollutant with strong immunotoxicity effects. Despite this, the mechanisms by which Cd causes spleen damage in chickens are not well understood. Astilbin (ASB) is a dihydroflavone glycoside with anti-inflammatory and anti-oxidation properties. In the present study, a chicken spleen injury model induced by cadmium exposure (90d) was established to explore the specific mechanisms of Cd-induced spleen injury. If and how ASB ameliorates the damage was also explored. A total of 60 chickens were randomly divided into four groups: Con, Cd, ASB, and Cd+ASB groups. The pathological changes in the spleen were observed by H&E staining. Cd-induced oxidative stress, inflammation, and the involvement of the MAPK/NF-κB/NLRP3 in ameliorating spleen damage were also analyzed by Western blotting, qRT-PCR, and immunohistochemistry. Our results showed that Cd exposure for 90 days damaged the spleen, which mainly manifested as eosinophil infiltration, an increase in MDA content, a decrease in the CAT, GSH, SOD, and T-AOC, and activation of MAPK/NF-κB/NLRP3 signaling pathway. The overall outcome of these events was the induction of oxidative stress and inflammation in the spleen of the chickens. Interestingly, ASB treatment ameliorated Cd-induced damages. In conclusion, the present study revealed the specific mechanism of Cd-induced spleen damage using a chicken model. But ASB ameliorates Cd-induced cadmium poisoning.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.