VvABF5 promotes ABA-mediated chlorophyll degradation by transcriptional activation of VvSGR during rachis browning of grape

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-12-13 DOI:10.1016/j.postharvbio.2024.113363

Zhiqian Li , Dongfang Zou , Changjiang Nie , Xing Chen , Chang He , Xianbo Zheng , Bin Tan , Jun Cheng , Wei Wang , Pedro Martínez Gómez , Xia Ye , Jiancan Feng

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引用次数: 0

Abstract

Rachis browning is a vital problem during postharvest storage of grape, and chlorophyll degradation is a main aspect of rachis browning. Uncovering the molecular mechanisms controlling the rachis browning process is essential to providing high quality grapes to the market. During rachis browning, the content of ABA increased, and treatment with nordihydroguaiaretic acid (NDGA) inhibited while treatment with ABA enhanced this process. A gene encoding a Stay-green protein (VvSGR) was highly expressed during rachis browning, and its overexpression in Arabidopsis and tobacco accelerated chlorophyll degradation, which suggests that VvSGR plays an important role in chlorophyll degradation. Yeast one-hybrid (Y1H), Dual-luciferase (Dual-LUC) and EMSA assays revealed that the transcription factor VvABF5 positively regulated expression of VvSGR by binding to a TCGT sequence in its promoter. Transient overexpression of VvABF5 in grape leaves resulted in a yellow phenotype and chlorophyll degradation. With overexpression of VvABF5, the expression of VvSGR was increased. Collectively, our findings revealed the ABA-responsive VvABF5-VvSGR module that regulates chlorophyll catabolism and the molecular mechanism underpinning rachis browning in grape.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.