Role of benzothiadiazole in regulating reactive oxygen species and inhibiting cap opening in Agaricus bisporus during storage

IF 6.4 1区 农林科学 Q1 AGRONOMY
Ruirui Yang , Yanan Wei , Hui Peng , Ting Wang , Jianmin Yun , Yan Yin , Yang Bi
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引用次数: 0

Abstract

Cap opening is an important aspect of the senescence process in Agaricus bisporus. Benzothiadiazole (BTH), a salicylic acid analogue, is known to induce plant resistance, but its effects on cap opening remain unexplored. This study investigates the effect of BTH on cap opening in A. bisporus (cv. A15). Mushrooms were sprayed with 200 mg/L BTH at the primordium stage. Changes in cap opening rate, cell membrane integrity, reactive oxygen species (ROS) production and scavenging mechanisms, respiration rate, weight loss, surface color and browning index were then monitored at harvest and during storage. The results showed that BTH treatment significantly reduced the cap opening rate during storage. This effect was associated with a decrease in NADPH oxidase (NOX) activity and an increase in the activities of superoxide dismutase (SOD) and catalase (CAT), together with increased levels of thioredoxin (Trx). BTH also increased the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), leading to increased levels of ascorbic acid (AsA) and oxidized glutathione (GSSG). These changes contributed to the inhibition of O2.- and H2O2 production, reduced cell membrane permeability and decreased malondialdehyde levels. BTH significantly reduced the respiratory rate and weight loss of the mushrooms. In addition, BTH also increased the L*, reduced a*, b*, ∆E and the browning index of A. bisporus. In conclusion, preharvest spraying of BTH effectively inhibited postharvest cap opening in A. bisporus by modulating ROS homeostasis, reducing respiration rates and weight loss. BTH delayed the browning of A. bisporus and maintained its quality. This study elucidates part of the mechanism by which BTH inhibits A. bisporus cap opening and provides an effective control measure to prevent A. bisporus cap opening.
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来源期刊
Postharvest Biology and Technology
Postharvest Biology and Technology 农林科学-农艺学
CiteScore
12.00
自引率
11.40%
发文量
309
审稿时长
38 days
期刊介绍: The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.
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