{"title":"PEI-Mediated Copper Deposition Strategy for Optical Signal Amplification on Lateral Flow Immunoassay Platform","authors":"Mengyao Li, Yao Wang* and Hong Xu, ","doi":"10.1021/acsaom.4c0045810.1021/acsaom.4c00458","DOIUrl":null,"url":null,"abstract":"<p >Here we report PEI-mediated copper in situ deposition (CISD) as a general signal enhancement strategy for a commercial colloidal gold nanoparticles (AuNPs)-based lateral flow immunoassay (LFIA) platform. The copper shell could be coated on AuNPs which aggregate on the test line to form core–shell structured composites (AuNPs@Cu) to deepen the colorimetric signal of the strips. After critical elements that affect Cu deposition yields were optimized, a significant signal amplification has been acquired with limit of detection (LOD) improvements of 40 times by naked-eye identification and 100 times by quantitative instrumental analysis using human hemoglobin (hHb) as a target. This study demonstrates a common and simple strategy for upgrading the detection sensitivity of a commercial AuNPs-LFIA platform with a whole running time of 20 min.</p>","PeriodicalId":29803,"journal":{"name":"ACS Applied Optical Materials","volume":"3 1","pages":"1–5 1–5"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Optical Materials","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsaom.4c00458","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Here we report PEI-mediated copper in situ deposition (CISD) as a general signal enhancement strategy for a commercial colloidal gold nanoparticles (AuNPs)-based lateral flow immunoassay (LFIA) platform. The copper shell could be coated on AuNPs which aggregate on the test line to form core–shell structured composites (AuNPs@Cu) to deepen the colorimetric signal of the strips. After critical elements that affect Cu deposition yields were optimized, a significant signal amplification has been acquired with limit of detection (LOD) improvements of 40 times by naked-eye identification and 100 times by quantitative instrumental analysis using human hemoglobin (hHb) as a target. This study demonstrates a common and simple strategy for upgrading the detection sensitivity of a commercial AuNPs-LFIA platform with a whole running time of 20 min.
期刊介绍：
ACS Applied Optical Materials is an international and interdisciplinary forum to publish original experimental and theoretical including simulation and modeling research in optical materials complementing the ACS Applied Materials portfolio. With a focus on innovative applications ACS Applied Optical Materials also complements and expands the scope of existing ACS publications that focus on fundamental aspects of the interaction between light and matter in materials science including ACS Photonics Macromolecules Journal of Physical Chemistry C ACS Nano and Nano Letters.The scope of ACS Applied Optical Materials includes high quality research of an applied nature that integrates knowledge in materials science chemistry physics optical science and engineering.
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