Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-01-01 DOI:10.1016/j.jobcr.2025.01.001

Marcelo Germani , Márcia V.G.B. de Queiroz , Mariana Yuri de França Shimizu , Thiago Gomes Teixeira , Victor Rogerio , Gabriela Giro , Victor R.M. Munoz-Lora

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引用次数: 0

Abstract

Objectives

This study aimed to compare the in-vitro behavior of four Hyaluronic acid (HA) gels when exposed to two different hyaluronidase (HSE) formulations.

Methods

Four commercially available HA were used: Lyft (Lt; Restylane, Galderma, Sweden), Voluma (Vol; Allergan, AbbVie, USA), UltraDeep (UDe; Rennova, Innovapharma, Brazil), and Subskin (Skn; Perfectha, Sinclair, France). The gels were divided into two groups, with seven aliquots (0.1 mL per aliquot) for each group deposited on a glass plate. A millimeter ruler was positioned behind the plate to measure the gel height. Each aliquot received 100 UTR of one of two HSE formulations. After 2 min, each aliquot was mixed using a 22G needle and left to sit for additional 2 min.

Results

Partial and final degradation of the products were obtained through photographic analysis. The final percentage of degradation exhibited significant differences among HA gels (P < 0.001). The results revealed higher degradation in Lt, moderate degradation in UDe, and lower degradation in Vol and Skn. Comparisons of HSE formulations showed no significant differences among them (P = 0.881). Moreover, there was a noticeable degradation after mixing (P < 0.001).

Conclusions

Within the limits of this study, it can be suggested that Lt exhibits the higher degradation among the experimented gels. Furthermore, differences among HSE formulations do not appear to significantly impact HA degradation, while the mixing movement of HSE and HA seems to influence the degradation rate. These findings may help guide clinical decisions regarding the use of hyaluronidase in managing HA filler complications or adjustments.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.