Cuscuta epithymum extract affects epileptic offspring behaviour and weight after applying preconception immobility stress on parents

IF 1.7 4区医学 Q3 DEVELOPMENTAL BIOLOGY

International Journal of Developmental Neuroscience Pub Date : 2025-02-03 DOI:10.1002/jdn.10405

Narjes Khavasi, Leila Ghassemifard, Hajar Ramezanikhah, Saeed Sardari, Ehsan Saboory

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引用次数: 0

Abstract

Introduction

Studies have indicated a link between stress during pregnancy and stress reactivity in offspring. In this study, we investigated the effect of Cuscuta epithymum (CE) extract on seizure and body weight in offspring born to stressed parents (pre-pregnancy).

Methods

Male and female rats were randomly separated into four groups. Then, the animals were subjected to restraint stress once a day for 50 (male) and 15 (female) continuous days. At the same time, rats in the control and stress groups received normal saline while the other two groups received the CE extract. After the stress induction, the control and experimental rats were mated to create eight types of coupling combinations as follows: McFc, McFs, MsFc, MsFs, McFc + EX, McFs + EX, MsFc + EX and MsFs + EX (M, male; F, female; C, control; S, stress; EX, extract). Then, the pups were weighed on postnatal days (PND) 1, 15 and 25. They were also evaluated for pentylenetetrazol (PTZ)-induced seizure on PND 25.

Results

The results showed that prolonged parental exposure to stress led to a significant increase in the duration and number of tonic–clonic (TC) seizures, duration of standing on two legs, firmly clinging to the ground, number of head nodding, total score of seizure behaviours and duration of tail rigidity in offspring. Moreover, the onset of the first convulsive behaviour was accelerated. Furthermore, a significantly lower body weight was observed in pre-conceptionally stressed offspring. However, the CE extract significantly improved these symptoms.

Conclusion

It can be concluded that CE administration possibly improves the consequences of pre-pregnancy stress, as well as seizure behaviours and weight loss in pups.

Abstract Image

查看原文本刊更多论文

胎皮上皮提取物对父母施加孕前静止应激后癫痫子代行为和体重的影响。

研究表明，怀孕期间的压力与后代的应激反应之间存在联系。在本研究中，我们研究了菟穗子上皮（CE）提取物对应激父母（孕前）所生后代癫痫发作和体重的影响。方法：将雄性、雌性大鼠随机分为4组。然后，每天1次进行约束应激，连续50 d（雄性）和15 d（雌性）。同时，对照组和应激组大鼠给予生理盐水，另外两组大鼠给予CE提取物。应激诱导后，将对照大鼠与实验大鼠进行配对，形成McFc、McFs、MsFc、MsFs、McFc + EX、McFs + EX、MsFc + EX和MsFs + EX 8种偶联组合(M，雄性；F,女;C、控制;年代,压力;前,提取)。然后，在出生后第1、15和25天称重。在PND 25日对他们进行戊四唑（PTZ）诱发的癫痫发作评估。结果：父母长时间的应激暴露导致子代强直-慢性发作的持续时间和次数、两腿站立时间、牢牢地抓地时间、点头次数、发作行为总分和尾僵持续时间显著增加。此外，第一次抽搐行为的发生也加快了。此外，孕前应激的后代体重显著降低。然而，CE提取物显著改善了这些症状。结论：CE给药可能改善孕前应激的后果，以及癫痫发作行为和幼犬体重减轻。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Developmental Neuroscience 医学-发育生物学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： International Journal of Developmental Neuroscience publishes original research articles and critical review papers on all fundamental and clinical aspects of nervous system development, renewal and regeneration, as well as on the effects of genetic and environmental perturbations of brain development and homeostasis leading to neurodevelopmental disorders and neurological conditions. Studies describing the involvement of stem cells in nervous system maintenance and disease (including brain tumours), stem cell-based approaches for the investigation of neurodegenerative diseases, roles of neuroinflammation in development and disease, and neuroevolution are also encouraged. Investigations using molecular, cellular, physiological, genetic and epigenetic approaches in model systems ranging from simple invertebrates to human iPSC-based 2D and 3D models are encouraged, as are studies using experimental models that provide behavioural or evolutionary insights. The journal also publishes Special Issues dealing with topics at the cutting edge of research edited by Guest Editors appointed by the Editor in Chief. A major aim of the journal is to facilitate the transfer of fundamental studies of nervous system development, maintenance, and disease to clinical applications. The journal thus intends to disseminate valuable information for both biologists and physicians. International Journal of Developmental Neuroscience is owned and supported by The International Society for Developmental Neuroscience (ISDN), an organization of scientists interested in advancing developmental neuroscience research in the broadest sense.