Evaluation of the DiaSorin NxTAG gastrointestinal pathogen panel (GPP) and its integration into routine diagnostics

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-01-29 DOI:10.1016/j.diagmicrobio.2025.116717

Raimond Lugert, Yvonne Laukat, Sabine Rabenda, Michael Weig, Uwe Groß, Wolfgang Bohne

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Abstract

This study evaluated the performance of the DiaSorin NxTAG GPP multiplex PCR system, the advanced successor of the Luminex-xTAG-GPP, in comparison to classical diagnostic techniques for stool samples. In a testing phase, we investigated 505 specimens using both the DiaSorin system and our conventional approach consisting of culture techniques, PCR detection and microscopic analysis. The multiplex PCR system proved to be superior in sensitivity, time efficiency, and pathogen range. The analysis of 2,462 stool samples after the system's implementation into routine diagnostics further confirmed its effectiveness. However, we encountered several Salmonella-positive samples, which could neither be confirmed by culture techniques nor by an independently performed Salmonella-specific PCR. This let us to permanently incorporate a confirmatory PCR assay for Salmonella-positive samples, in order to avoid false positives. Our findings indicate that the DiaSorin NxTAG GPP is a reliable and efficient diagnostic tool for most gastrointestinal pathogens, with the exception of Salmonella.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.