{"title":"Polymorphism in the <i>MLANA</i> gene and its association with feather pigmentation in Chinese yellow quail (<i>Coturnix japonica</i>).","authors":"X Zhang, F Wu, J Hu, X Ji, Y Qi","doi":"10.1080/00071668.2025.2451243","DOIUrl":null,"url":null,"abstract":"<p><p>1. Melan-A <i>(MLANA)</i> plays a key role in the development of the melanosome, making it a strong candidate for the pigmentation phenotype observed in animals. The main purpose of this study was to analyse the relationship between <i>MLANA</i> gene polymorphisms and tyrosinase (TYR) enzyme activity in skin tissues and melanin content in dorsal down feathers of Chinese yellow quail.2. The coding sequence region of <i>MLANA</i> mRNA was cloned and sequenced to detect polymorphisms. The melanin content in down feathers of 266 Chinese yellow quails was analysed by spectrophotometry, and TYR enzyme activity was measured in dorsal skin tissues. The expression of <i>MLANA</i> mRNA in skin tissues of individuals with different genotypes was analysed using RT-qPCR.3. One non-synonymous single nucleotide polymorphisms (NSSNP; c.218T/A) was identified, which resulted in a Leu36Val mutation in the transmembrane helix region of the MLANA protein. This NSSNP significantly reduced the expression level of <i>MLANA</i> mRNA and TYR enzyme activity in dorsal skin tissues, leading to a significant reduction in melanin content in down feathers.4. The c.218T/A locus of the <i>MLANA</i> gene is closely related to the pigmentation TYR of the down feathers in Chinese yellow quail and can be used as a molecular marker locus for breeding pure feather colour in quail.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-5"},"PeriodicalIF":1.6000,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2025.2451243","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
1. Melan-A (MLANA) plays a key role in the development of the melanosome, making it a strong candidate for the pigmentation phenotype observed in animals. The main purpose of this study was to analyse the relationship between MLANA gene polymorphisms and tyrosinase (TYR) enzyme activity in skin tissues and melanin content in dorsal down feathers of Chinese yellow quail.2. The coding sequence region of MLANA mRNA was cloned and sequenced to detect polymorphisms. The melanin content in down feathers of 266 Chinese yellow quails was analysed by spectrophotometry, and TYR enzyme activity was measured in dorsal skin tissues. The expression of MLANA mRNA in skin tissues of individuals with different genotypes was analysed using RT-qPCR.3. One non-synonymous single nucleotide polymorphisms (NSSNP; c.218T/A) was identified, which resulted in a Leu36Val mutation in the transmembrane helix region of the MLANA protein. This NSSNP significantly reduced the expression level of MLANA mRNA and TYR enzyme activity in dorsal skin tissues, leading to a significant reduction in melanin content in down feathers.4. The c.218T/A locus of the MLANA gene is closely related to the pigmentation TYR of the down feathers in Chinese yellow quail and can be used as a molecular marker locus for breeding pure feather colour in quail.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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