Development and characterization of potential antibacterial biocomposites: Lysozyme-loaded cellulose-alginate materials

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-25 DOI:10.1016/j.colsurfb.2025.114536

Carlos Eduardo Camacho-González , Alejandro Pérez-Larios , Cesar S. Cardona-Félix , Sonia G. Sáyago-Ayerdi , Víctor M. Zamora-Gasga , Ulises M. López-García , Jorge A. Sánchez-Burgos

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引用次数: 0

Abstract

The objective of this study was to design and characterize novel biocomposites based on modified cellulose/alginate oligosaccharides loaded with hen egg lysozyme (CLm/AOS/Lyz) as a potential alternative to combat bacterial proliferation. An antimicrobial enzyme, lysozyme, was immobilized within polymeric matrices to enhance its bactericidal capacity and stability. The biocomposites synthesized at pH levels 3, 5, and 8 (CLm/AOS/Lyz₃, CLm/AOS/Lyz₅, and CLm/AOS/Lyz₈, respectively) were analyzed using Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), and energy-dispersive X-ray spectroscopy (EDS). Results demonstrated successful lysozyme conjugation to the biocomposite without altering its secondary structure or stability. The biocomposites exhibited irregular morphologies and strong adhesion between CLm/AOS and Lyz, with CLm/AOS/Lyz5 showing the highest nitrogen composition and protein content (2086.43 ± 100.90 µg of bovine serum albumin equivalents). Antibacterial assays revealed significant log reductions in viable E. faecalis cells for CLm/AOS/Lyz₃ and CLm/AOS/Lyz₅ (5.72 ± 0.17 and 5.78 ± 0.24 respectively), concerning the blank (8.04 ± 0.07), even comparable to free lysozyme (5.85 ± 0.35). However, no reduction in viable cell counts was observed for Gram-negative bacteria. This work highlights the potential of lysozyme-loaded cellulose-alginate biocomposites as novel antibacterial agents for effective applications in pharmaceutical and food technology fields.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.