Phagocytosis and reactive oxygen species (ROS) production by CH138+ granulocytes isolated from blood, colostrum, and milk of Holstein cows during transition period

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-01-26 DOI:10.1016/j.vetimm.2025.110888

Cynthia Pereira da Costa e Silva , Karina Médici Madureira , Vinicius Alvim Passos Baldacim , Juliana França dos Reis Costa , Bianca Paola Santarosa , Cristina de Oliveira Massoco Salles Gomes , Viviani Gomes

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Abstract

This study investigated the functional activity of CH138 + granulocytes in the blood, colostrum, and transitional milk of Holstein cows throughout the transition period. Thirteen cows were assessed weekly, beginning three weeks before calving (W-3, W-2, and W-1), on the day of calving, and continuing to three weeks postpartum (W1, W2, and W3). Physical examination of the udder tissue, bacterial cultures, and blood immune function tests were performed. Microscopic Somatic Cell Count (MSCC), phagocytosis, and intracellular ROS production by CH138 + granulocytes in colostrum and transitional milk were measured weekly, from calving to W3. Disease incidence was recorded, with four cows developing metritis at W2. Mammary gland edema, affecting parenchymal tissue consistency, was the main physical finding. Non-Aureus Staphylococcus spp. dominated the bacterial isolates from the mammary secretions (67.5 %). MSCC was highest in colostrum and decreased postpartum. Bacterial culture rates peaked at calving; however, colostrogenesis limited the detection of clinical mastitis through specific examinations. Blood neutrophil function decreased at W-2 and W-1, likely increasing the risk of mammary infection and weakening the neutrophil response at calving. This immune suppression, which affects both the mammary gland and systemic immunity, was exacerbated by postpartum metritis. The findings showed heightened vulnerability to bacterial infection postpartum, which was linked to reduced cell viability and CH138 + granulocyte function. Neutrophil function in mammary secretions gradually improves post-calving, supporting immune recovery and declining infection rates. These findings provided valuable insights into the immune adaptations of CH138 + granulocytes in colostrum and transitional milk, enhancing understanding of mammary and systemic immune responses during the transition period.

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.