The neuroprotective effect of elderberry diet on the tramadol-induced toxicity in the hippocampus of adult male rats

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-01-27 DOI:10.1016/j.tice.2025.102757

Keysan Sohrabi , Ibrahim Mohammadzadeh , Seyedeh Mah Sa Kiaeipour Siahkal , Mohammad Javad Ebrahimi , Maral Moafi , Negin Adimi , Sina Dolatshahi , Amirreza Beirami , Maral Hasanzadeh , Alireza joudaki , Soheil Taherpouran , Siavash Parvardeh , Gholam Hossein Meftahi , Mohammadamin Sabbaghalvani , Susan Roustaee , Ghazal khanjari , Haleh Hemmatparast , Mojtaba Sani , Meysam Hassani Moghaddam , Abbas Aliaghaei

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引用次数: 0

Abstract

Pain relievers such as tramadol are among the most commonly prescribed around the world. However, Long-term tramadol use has been shown to cause neurotoxicity, neuroinflammation, and cognitive impairment, which has limited its clinical use. Elderberry (EB), a plant extract widely recognized for its medicinal properties, is used in this study to investigate the effects of a plant-based diet on tramadol's adverse effects in the hippocampus region. The effects of EB oral diet on the devastating effects of tramadol on the hippocampus structure and function of 36 male albino rats were evaluated using behavioral, electrophysiological, and histological setups. Three groups (Control, Tramadol, and Tramadol+EB) were examined using behavioral, electrophysiological, and histological setups. The behavioral findings showed that the EB diet could improve most of the memory-related indices in Tramadol+EB compared to the Tramadol group. Regarding the fEPSP slope and population spike amplitude, these electrophysiological parameters returned to control levels in the Tramadol+EB group. Additionally, a decrease in caspase-3 expression was observed in hippocampal cells. While there was a reduction in astrogliosis in the Tramadol+EB group compared to the Tramadol group, no significant differences were noted in the morphological variations of astrocytes between the groups. In conclusion, although further studies are necessary to elucidate other aspects of EB's effects on the brain, it may be considered a potential neuroprotective agent in acute to sub-chronic tramadol exposure.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.