Cerebrospinal fluid multiplex PCR cycle thresholds may predict ICU admission in community-acquired meningoencephalitis

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-01-27 DOI:10.1016/j.diagmicrobio.2025.116704

Pilar Vizcarra, David Grandioso Vas, María Inmaculada Quiles Melero, Juana Cacho Calvo, Emilio Cendejas Bueno

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Abstract

Objective

We investigated the relationship between cerebrospinal fluid (CSF) PCR cycle threshold (Ct) values, clinical characteristics, and outcomes in suspected CAM, while assessing QIAstat-Dx® ME Panel concordance with routine diagnostic methods.

Methods

Frozen CSF from 30 individuals with suspected CAM were analyzed using the QIAstat-Dx® ME Panel. Ct values were categorized as low (≤ 30) or high (>30). Concordance with CSF FilmArray®/culture results was assessed using the Kappa coefficient.

Results

Low Ct values were associated to CSF markers of meningitis and predicted ICU admissions (log-rank P = 0.025), particularly within the first two weeks of hospitalization (85 % for low Ct values versus 50 % for high Ct values, P = 0.041). Ct values were not associated with mortality in the survival analysis (log-rank P = 0.109). Substantial agreement was observed between QIAstat-Dx® and comparators (96.7 %, Kappa 0.839).

Conclusions

CSF Ct values can assist in risk stratification for timely ICU admission in individuals with CAM.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.