Brooke L Boger, Marc A Kinsley, Chelsey J Yob, Lindsay E Knott, Jane M Manfredi
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引用次数: 0
Abstract
Objective: To determine if equine platelet-rich plasma (PRP) contains Dickkopf-1 (Dkk-1) and, if so, to quantify the amount of Dkk-1 in bovine thrombin (BT)-activated PRP and a control platelet lysate created using a freeze-thaw (FT) method.
Methods: 10 adult horses were used in this experimental study. Blood was collected and processed in November 2019 and stored at -80 °C until the ELISAs were performed in October of 2023. Blood was collected from each horse, and PRP was created using a commercially available kit. Known concentrations of platelets were activated using BT or by an FT cycle to create platelet lysates. Dickkopf-1 and platelet-derived growth factor concentrations were measured using ELISAs. Statistics included the Shapiro-Wilks test, Student t tests, linear regression, and Pearson or Spearman correlations (significant at P ≤ .05).
Results: Bovine thrombin created a greater release of Dkk-1 from platelets (250.22 ± 129.09 pg/mL from the combined clot and releasate) than the FT cycle (152.81 ± 62.74 pg/mL). There was a positive correlation between platelet concentration and Dkk-1 for both the FT (r = 0.69) and the BT activation method (r = 0.62). Platelet-derived growth factor was released in FT- and BT-activated PRP (FT = 530.35 ± 253.96 pg/mL; BT = 1391.05 ± 661.50 pg/mL).
Conclusions: Dickkopf-1 is present in equine PRP and is correlated with platelet concentration. Although more investigation of the active proteins in PRP is needed, this study provides the first evidence that equine platelets contain Dkk-1.
Clinical relevance: Equine PRP contains Dkk-1, which could contribute to PRP's mechanism of action.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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