Transcriptome Sequencing Reveals Effects of Artificial Feed Domestication on Intestinal Performance and Gene Expression of Carnivorous Mandarin Fish (Siniperca chuatsi) and Related Mechanisms

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jia-Xing Luo, Xiao-Tian Gao, Zhen Rong, Li-Han Zhang, Yan-Feng Sun, Zun-Li Qi, Qi Yu, Khor Waiho, Wei-Xu Zhao, Yi-Huan Xu, Chun-Long Zhao, Cheng-Bin Wu
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引用次数: 0

Abstract

Mandarin fish (Siniperca chuatsi) is a voracious carnivorous species, usually consuming only live bait fish, but dietary acclimation enables it to accept artificial feed. However, the effects of dietary acclimation on intestinal performance and gene expression in mandarin fish and related mechanisms remain largely unknown. Therefore, this study investigated the effects of artificial feed on intestinal physicochemical and biochemical performance and gene expression in mandarin fish. Mandarin fish were sampled on day 10 after feeding with live dace (LD), at day 40 after subsequent feeding with dead dace plus artificial feed (DD + AF) from day 11 to day 40, and at day 90 after continuous feeding with artificial feed (AF) alone from day 41 to day 90 for transcriptome sequencing. The biochemical analysis results indicated that artificial feed significantly increased the activity of antioxidant enzymes glutathione peroxidase and superoxide dismutase in the intestine, liver, and stomach. Histological analysis demonstrated intestinal damage in mandarin fish fed with artificial feed. The GO and KEGG enrichment analyses indicated that the DEGs in AF vs. DD + AF were significantly enriched in the pentose phosphate pathway, and the DEGs in AF vs. LD were mainly significantly enriched in glycolysis/gluconeogenesis and PPAR signaling pathways. Nineteen feed acclimation–related key genes such as gene pfkfb4a and scd were identified in the intestine and found to exhibit upregulated expressions. These results revealed that artificial feed domestication enhanced the antioxidant capacity of the mandarin fish intestine and reduced hepatic lipid deposition by upregulating the related gene expression of mandarin fish and that the regulation of carbon metabolisms, including sugar, lipid, and steroid metabolisms, might be fundamental mechanisms for mandarin fish to acclimatize to dietary changes. These findings provide novel insights into the feed acclimation mechanism of mandarin fish, holding implications for promoting large-scale artificial feed aquaculture of mandarin fish and improving economic efficiency.

转录组测序揭示人工饲料驯化对肉食性鳜鱼肠道性能和基因表达的影响及其机制
鳜鱼(sininiperca chuatsi)是一种贪婪的肉食性鱼类,通常只食用活饵料鱼,但饮食适应使其能够接受人工饲料。然而,饲料驯化对鳜鱼肠道性能和基因表达的影响及其相关机制尚不清楚。为此,本研究旨在探讨人工饲料对鳜鱼肠道理化生化性能及基因表达的影响。分别于第10天饲喂活鲮鱼(LD),第11天至第40天饲喂死鲮鱼加人工饲料(DD + AF),第41天至第90天连续饲喂人工饲料(AF),第40天取样进行转录组测序。生化分析结果表明,人工饲料显著提高了肠道、肝脏和胃中抗氧化酶谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。组织学分析表明,饲喂人工饲料对鳜鱼的肠道有损伤。GO和KEGG富集分析表明,AF与DD + AF中的DEGs在戊糖磷酸途径中显著富集,AF与LD中的DEGs主要在糖酵解/糖异生和PPAR信号通路中显著富集。在肠道中鉴定出19个与饲料驯化相关的关键基因,如pfkfb4a和scd基因,并发现其表达上调。上述结果表明,人工饲料驯化通过上调鳜鱼的相关基因表达,增强了鳜鱼肠道的抗氧化能力,减少了肝脏脂质沉积,而糖、脂质和类固醇代谢等碳代谢的调节可能是鳜鱼适应饲料变化的根本机制。这些研究结果为鳜鱼饲料驯化机制的研究提供了新的思路,对促进鳜鱼大规模人工饲料养殖和提高经济效益具有重要意义。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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