Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-01-31 DOI:10.1016/j.biotechadv.2025.108523

Maša Vodovnik , Nataša Lindič

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Abstract

Cellulosomes are highly efficient, complex multi-enzyme assemblies, predominantly found in anaerobic bacteria, which offer substantial potential for second-generation biofuel production through efficient lignocellulosic biomass degradation, thus reducing the need for costly pretreatments. Recent advances in cellulosome research have significantly contributed to developing more efficient consolidated bioprocessing (CBP) platforms for biofuel production. This review highlights the latest progress in designing cellulosomes for optimized enzyme synergy and substrate specificity, as well as advances in engineering cellulosome-producing whole-cell biocatalysts tailored for biofuel applications. Apart from recombinant approaches to the development of CBP platforms, metabolic engineering of cellulosome-producing strains (CPS) and co-culture systems that combine CPS with solvent-producing microbes are also discussed. Current challenges and future directions are outlined that emphasize the role of cellulosomes as powerful tools in advancing the efficiency of lignocellulosic biorefineries.

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迈向自然界催化纳米机器的应用：第二代生物燃料生产中的纤维素

纤维素体是高效、复杂的多酶组件，主要存在于厌氧细菌中，通过有效的木质纤维素生物质降解，为第二代生物燃料的生产提供了巨大的潜力，从而减少了昂贵的预处理需求。纤维素研究的最新进展为开发更有效的生物燃料生产综合生物处理（CBP）平台做出了重大贡献。本文综述了优化酶协同作用和底物特异性的纤维素体设计的最新进展，以及用于生物燃料应用的工程纤维素体生产全细胞生物催化剂的进展。除了重组方法开发CBP平台外，还讨论了产纤维素菌（CPS）的代谢工程以及将CPS与产溶剂微生物结合的共培养系统。概述了当前的挑战和未来的方向，强调了纤维素作为提高木质纤维素生物炼制效率的有力工具的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.