sFlt-1, Coagulation Function, and Platelets as Predictors of Preeclampsia

IF 2 Q2 OBSTETRICS & GYNECOLOGY

Journal of obstetrics and gynaecology Canada Pub Date : 2025-01-28 DOI:10.1016/j.jogc.2025.102772

Jiani Yuan MM, Duanqing Wu BM, Jun Ye MM, Rujun Chen MM, Xiaoqin Wang MM, Liwen Zhang BM

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引用次数: 0

Abstract

Objective

To investigate the predictive value of soluble FMS-like tyrosine kinase-1 (sFlt-1), coagulation function, and platelet (PLT) parameters for preeclampsia (PE).

Methods

A prospective study was conducted on women registered and delivered at Shanghai Fifth People's Hospital from October 2020 to December 2021. All eligible pregnant women were recruited at the time of initial registration in the first trimester. We then obtained serum samples uniformly at 24⁰–28⁰ weeks and stored these samples in a freezer at −80°C and labelled them to create a biobank. Later, when PE was diagnosed, we followed the markers to find their blood samples and complete the tests. Participants were divided into healthy pregnant (HP) and PE groups. Participants were divided into HP and PE groups. Approximately 5 mL of venous blood was collected from each participant at 24⁰–28⁰ weeks gestation. Serum sFlt-1 was measured by enzyme-linked immunosorbent assay. Additionally, D-dimer, activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), antithrombin III (ATIII), fibrinogen, PLT, PLT distribution width (PDW), and mean PLT volume (MPV) were recorded. SPSS 27.0 software was used to analyze the correlation of these parameters with PE. Receiver operating characteristic curve analysis determined the optimal cutoff value for each parameter.

Results

Serum sFlt-1, APTT, TT, ATIII, PLT, MPV, and PDW levels were significantly different between the PE and HP groups (P < 0.05). Among single-factor indicators for predicting PE, sFlt-1 exhibited the highest value. With an optimal cutoff value of 4.409 ng/mL, sFlt-1 demonstrated a sensitivity and specificity of 85.4% and 87.5%, respectively. The combination of sFlt-1, APTT, TT, PDW, and MPV yielded the highest predictive value, with an area under the receiver operating characteristic curve of 0.946, sensitivity of 86.8%, and specificity of 87.5%.

Conclusions

This study demonstrates that a combination of sFlt-1, APTT, TT, PDW, and MPV is a valuable tool for predicting PE.

查看原文本刊更多论文

sFlt-1、凝血功能和血小板作为先兆子痫的预测因子。

目的：探讨可溶性fms样酪氨酸激酶-1 （sFlt-1）、凝血功能和血小板（PLT）参数对子痫前期（PE）的预测价值。方法：对2020年10月至2021年12月在XXXX医院登记分娩的妇女进行前瞻性研究。所有符合条件的孕妇都是在妊娠早期首次登记时招募的。然后，我们在24-28周均匀地获得血清样本，并将这些样本保存在-80°C的冰箱中，并标记它们以创建生物库。后来，当PE被确诊时，我们根据标记物找到他们的血液样本并完成测试。参与者分为健康孕妇组（HP）和健康孕妇组（PE）。参与者分为健康孕妇组（HP）和健康孕妇组（PE）。在妊娠24-28周时从每位参与者身上采集约5ml静脉血。采用酶联免疫吸附试验（ELISA）检测血清sFlt-1。此外，记录d -二聚体（D-D）、活化的部分凝血活素时间（APTT）、凝血酶时间（TT）、凝血酶原时间（PT）、抗凝血酶III （ATIII）、纤维蛋白原（FIB）、PLT、血小板分布宽度（PDW）和平均血小板体积（MPV）。采用SPSS 27.0软件分析这些参数与PE的相关性。受试者工作特征（ROC）曲线分析确定各参数的最佳截止值。结果：PE组与HP组血清sFlt-1、APTT、TT、ATIII、PLT、MPV、PDW水平差异均有统计学意义（P < 0.05）。在预测PE的单因素指标中，sFlt-1的预测值最高。sFlt-1的最佳截止值为4.409 ng/ml，敏感性为85.4%，特异性为87.5%。sFlt-1、APTT、TT、PDW和MPV联合检测的预测价值最高，ROC曲线下面积（AUC）为0.946，敏感性为86.8%，特异性为87.5%。结论：本研究表明sFlt-1、APTT、TT、PDW和MPV的组合是预测PE的有价值的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of obstetrics and gynaecology Canada OBSTETRICS & GYNECOLOGY-

CiteScore

3.30

自引率

5.60%

发文量

302

审稿时长

32 days

期刊介绍： Journal of Obstetrics and Gynaecology Canada (JOGC) is Canada"s peer-reviewed journal of obstetrics, gynaecology, and women"s health. Each monthly issue contains original research articles, reviews, case reports, commentaries, and editorials on all aspects of reproductive health. JOGC is the original publication source of evidence-based clinical guidelines, committee opinions, and policy statements that derive from standing or ad hoc committees of the Society of Obstetricians and Gynaecologists of Canada. JOGC is included in the National Library of Medicine"s MEDLINE database, and abstracts from JOGC are accessible on PubMed.