Investigating the role of adipose tissue in mobility and aging: design and methods of the Adipose Tissue ancillary to the Study of Muscle, Mobility and Aging (SOMMA-AT).

Abstract

Background: Age-related changes in adipose tissue impact chronic medical diseases and mobility disability but mechanism remains poorly understood. The goal of this study is to define methods for phenotyping unique characteristics of adipose tissue from older adults.

Methods: Older adults enrolled in Study of Muscle, Mobility and Aging selected for the adipose tissue ancillary (SOMMA-AT; N=210, 52.38% women, 76.12±4.37 years) were assessed for regional adiposity by whole-body magnetic resonance (AMRA) and underwent a needle-aspiration biopsy of abdominal subcutaneous adipose tissue (ASAT). ASAT biopsies were flash frozen, fixed, or processed for downstream applications and deposited at the biorepository. Biopsy yields, qualitative features, adipocyte sizes, and concentration of adipokines secreted in ASAT explant conditioned media were measured. Inter-measure Spearman correlations were determined.

Results: Regional, but not total, adiposity differed by sex: women had greater ASAT mass (8.20±2.73kg, p<0.001) and biopsy yield (3.44±1.81g, p<0.001) than men (ASAT=5.95±2.30kg, biopsy=2.30±1.40g). ASAT mass correlated with leptin (r=0.54, p<0.001) and not resistin (p=0.248) and adiponectin (p=0.353). Adipocyte area correlated with ASAT mass (r=0.34, p<0.001), BMI (r=0.33, p<0.001), adiponectin (r=-0.22, p=0.005) and leptin (r=0.18, p=0.024) but not with resistin (p=0.490).

Conclusion: In addition to the detailed ASAT biopsy processing in this report, we found that adipocyte area correlated with ASAT mass, and both measures related to some key adipokines in the explant conditioned media. These results, methods, and biological repositories underscore the potential of this unique cohort to impact the understanding of aging adipose biology on disease, disability, and other aging tissues.