RNA interference in protozoan parasites and its application

IF 3.7 2区 医学 Q2 IMMUNOLOGY
Lon-Fye Lye , Deborah E. Dobson , Stephen M. Beverley , Min-Che Tung
{"title":"RNA interference in protozoan parasites and its application","authors":"Lon-Fye Lye ,&nbsp;Deborah E. Dobson ,&nbsp;Stephen M. Beverley ,&nbsp;Min-Che Tung","doi":"10.1016/j.jmii.2025.01.005","DOIUrl":null,"url":null,"abstract":"<div><div><strong>RNA interference</strong> (<strong>RNAi</strong>) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression, via small RNA triggers derived from double-stranded RNA that can target specific genes; it is a natural process that plays a role in both the regulation of protein synthesis and in immunity. Discovery of RNAi by Fire and Mello in 1998 had a profound impact on unraveling novel aspects of eukaryotic biology. RNA interference (RNAi) has proven to be an immensely useful tool for studying gene function and validation of potential drug targets in almost all organisms. A great advance in parasitic protozoa was achieved by the experimental demonstration of RNAi in <em>Trypanosoma brucei</em>, and in other protists such as <em>Leishmania braziliensis, Entamoeba histolytica</em> and <em>Giardia lamblia/intestinalis</em>. These organisms exhibit numerous differences beyond the core ‘dicer’ and ‘slicer’ activities, thereby expanding knowledge of the evolutionary diversification of this pathway in eukaryotes. When present, RNAi has led to new technologies for engineering powerful and facile knockdowns in gene expression, revolutionizing biomedical research and opening clinical potentialities. In this review, we discuss the distribution of RNAi pathways, their biological roles, and experimental applications in protozoan parasites.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"58 3","pages":"Pages 281-287"},"PeriodicalIF":3.7000,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microbiology Immunology and Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1684118225000088","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

RNA interference (RNAi) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression, via small RNA triggers derived from double-stranded RNA that can target specific genes; it is a natural process that plays a role in both the regulation of protein synthesis and in immunity. Discovery of RNAi by Fire and Mello in 1998 had a profound impact on unraveling novel aspects of eukaryotic biology. RNA interference (RNAi) has proven to be an immensely useful tool for studying gene function and validation of potential drug targets in almost all organisms. A great advance in parasitic protozoa was achieved by the experimental demonstration of RNAi in Trypanosoma brucei, and in other protists such as Leishmania braziliensis, Entamoeba histolytica and Giardia lamblia/intestinalis. These organisms exhibit numerous differences beyond the core ‘dicer’ and ‘slicer’ activities, thereby expanding knowledge of the evolutionary diversification of this pathway in eukaryotes. When present, RNAi has led to new technologies for engineering powerful and facile knockdowns in gene expression, revolutionizing biomedical research and opening clinical potentialities. In this review, we discuss the distribution of RNAi pathways, their biological roles, and experimental applications in protozoan parasites.
原生寄生虫的RNA干扰及其应用。
RNA干扰(RNAi)是一种生物学过程,其中RNA分子通过来自双链RNA的小RNA触发器参与基因表达的序列特异性抑制,这些小RNA触发器可以靶向特定基因;这是一个自然过程,在调节蛋白质合成和免疫中都起作用。1998年Fire和Mello发现的RNAi对揭示真核生物的新方面产生了深远的影响。RNA干扰(RNAi)已被证明是研究基因功能和验证潜在药物靶点的一种非常有用的工具。通过对布鲁氏锥虫、巴西利什曼原虫、溶组织内阿米巴原虫和蓝氏贾第鞭毛虫等原生生物的实验证明,RNAi在寄生原生动物方面取得了很大进展。这些生物在核心的“掷骰子”和“切片”活动之外表现出许多差异,从而扩大了对真核生物这一途径进化多样化的认识。目前,RNAi已经导致了在基因表达中进行强大而容易的敲除的工程新技术,彻底改变了生物医学研究并打开了临床潜力。本文综述了RNAi途径的分布、生物学作用及其在原虫寄生虫中的实验应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Microbiology Immunology and Infection
Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES
CiteScore
15.90
自引率
5.40%
发文量
159
审稿时长
67 days
期刊介绍: Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信