Sister chromatid cohesion through the lens of biochemical experiments

IF 6 2区生物学 Q1 CELL BIOLOGY

Current Opinion in Cell Biology Pub Date : 2025-01-28 DOI:10.1016/j.ceb.2025.102464

Yasuto Murayama

引用次数: 0

Abstract

Faithful chromosome segregation in eukaryotes relies on physical cohesion between newly duplicated sister chromatids. Cohesin is a ring-shaped ATPase assembly that mediates sister chromatid cohesion through its ability to topologically entrap DNA. Cohesin, assisted by several regulatory proteins, binds to DNA prior to DNA replication and then holds two sister DNAs together when it encounters the replication machinery. Cohesion establishment further requires cohesin acetylation, which confers near eternal stability on chromatin-bound cohesin until the onset of chromosome segregation. In addition to a wealth of experimental evidence from cellular studies, recent advances in reconstitution approaches are now beginning to unravel the biochemical properties of cohesin that underlie its function in sister chromatid cohesion. This review summarizes recent insights into the mechanism of cohesion establishment.

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姐妹染色单体内聚通过镜头进行生化实验。

真核生物中忠实的染色体分离依赖于新复制的姐妹染色单体之间的物理内聚。内聚蛋白是一种环状atp酶组装体，通过其拓扑诱捕DNA的能力介导姐妹染色单体内聚。内聚蛋白在几种调节蛋白的协助下，在DNA复制之前与DNA结合，然后在遇到复制机制时将两个姊妹DNA结合在一起。内聚的建立进一步需要内聚蛋白乙酰化，这赋予染色质结合的内聚蛋白近乎永久的稳定性，直到染色体分离开始。除了来自细胞研究的大量实验证据外，重组方法的最新进展现在开始揭示黏结蛋白的生化特性，这些特性是其在姐妹染色单体黏结中功能的基础。本文综述了近年来对衔接机制的研究进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Cell Biology 生物-细胞生物学

CiteScore

14.60

自引率

1.30%

发文量

审稿时长

93 days

期刊介绍： Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.