Distinct checkpoint and homolog biorientation pathways regulate meiosis I in Drosophila oocytes.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-01-29 eCollection Date: 2025-01-01 DOI:10.1371/journal.pgen.1011400
Joanatta G Shapiro, Neha Changela, Janet K Jang, Jay N Joshi, Kim S McKim
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引用次数: 0

Abstract

Mitosis and meiosis have two mechanisms for regulating the accuracy of chromosome segregation: error correction and the spindle assembly checkpoint (SAC). We have investigated the function of several checkpoint proteins in meiosis I of Drosophila oocytes. Increased localization of several SAC proteins was found upon depolymerization of microtubules by colchicine. However, unattached kinetochores or errors in biorientation of homologous chromosomes do not induce increased SAC protein localization. Furthermore, the metaphase I arrest does not depend on SAC genes, suggesting the APC is inhibited even if the SAC is not functional. Two SAC proteins, ROD of the ROD-ZW10-Zwilch (RZZ) complex and MPS1, are also required for the biorientation of homologous chromosomes during meiosis I, suggesting an error correction function. Both proteins aid in preventing or correcting erroneous attachments and depend on SPC105R for localization to the kinetochore. We have defined a region of SPC105R, amino acids 123-473, that is required for ROD localization and biorientation of homologous chromosomes at meiosis I. Surprisingly, ROD removal from kinetochores and movement towards spindle poles, termed "streaming," is independent of the dynein adaptor Spindly and is not linked to the stabilization of end-on attachments. Instead, meiotic RZZ streaming appears to depend on cell cycle stage and may be regulated independently of kinetochore attachment or biorientation status. We also show that Spindly is required for biorientation at meiosis I, and surprisingly, the direction of RZZ streaming.

不同的检查点和同源双向通路调节果蝇卵母细胞减数分裂I。
有丝分裂和减数分裂有两种调节染色体分离准确性的机制:错误纠正和纺锤体组装检查点(SAC)。我们研究了几种检查点蛋白在果蝇卵母细胞减数分裂中的功能。秋水仙碱解聚微管后,发现一些SAC蛋白的定位增加。然而,未附着的着丝点或同源染色体的双向错误不会诱导SAC蛋白定位增加。此外,中期I阻滞并不依赖于SAC基因,这表明即使SAC不起作用,APC也会受到抑制。两种SAC蛋白,ROD- zw10 - zwilch (RZZ)复合体的ROD和MPS1,也需要在减数分裂I期间同源染色体的双向定位,表明其具有纠错功能。这两种蛋白都有助于防止或纠正错误的附着,并依赖于SPC105R定位到着丝点。我们已经定义了SPC105R的一个区域,氨基酸123-473,这是减数分裂i时同源染色体的ROD定位和双向定位所必需的。令人惊讶的是,ROD从着丝点移除并向纺锤极移动,称为“流”,是独立于动力蛋白适配器spendly的,与端上附件的稳定无关。相反,减数分裂RZZ流似乎依赖于细胞周期阶段,并可能独立于着丝点附着或双向状态进行调节。我们还发现,在减数分裂I中,Spindly是双向性所必需的,令人惊讶的是,RZZ流的方向也是如此。
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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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