Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan

IF 2.7 4区 医学 Q3 VIROLOGY
Yuki Nishisaka , Hikaru Fujii , Fumiko Ono , Sho Kadekaru , Hiroyuki Kogiku , Yumi Une , Shione Takeguchi , Naomi Ohta , Masumi Eto , Chiharu Takeuchi , Seigou Takeuchi , Tetsuko Miki , Akihiko Tokuda , Keiko Ookawa , Yukinobu Tohya , Keita Ishijima , Akiko Okutani , Ken Maeda , Shumpei Watanabe , Shigeru Morikawa
{"title":"Molecular characterization of feline caliciviruses isolated from several adult cats with atypical infection showing severe flu-like symptoms on a remote island in Ehime, Japan","authors":"Yuki Nishisaka ,&nbsp;Hikaru Fujii ,&nbsp;Fumiko Ono ,&nbsp;Sho Kadekaru ,&nbsp;Hiroyuki Kogiku ,&nbsp;Yumi Une ,&nbsp;Shione Takeguchi ,&nbsp;Naomi Ohta ,&nbsp;Masumi Eto ,&nbsp;Chiharu Takeuchi ,&nbsp;Seigou Takeuchi ,&nbsp;Tetsuko Miki ,&nbsp;Akihiko Tokuda ,&nbsp;Keiko Ookawa ,&nbsp;Yukinobu Tohya ,&nbsp;Keita Ishijima ,&nbsp;Akiko Okutani ,&nbsp;Ken Maeda ,&nbsp;Shumpei Watanabe ,&nbsp;Shigeru Morikawa","doi":"10.1016/j.virusres.2025.199535","DOIUrl":null,"url":null,"abstract":"<div><div>In November 2020, a volunteer group reported an outbreak of an infectious disease with a high fatality rate and flu-like symptoms among stray cats in Aoshima, a remote island in Ehime, Japan. Nine adult cats with severe symptoms were hospitalized. Feline calicivirus (FCV) was isolated from pharyngeal swabs of six hospitalized cats. An outbreak of virulent systemic FCV (VS-FCV) infection was initially suspected because of obvious flu-like symptoms in adult cats; however, no symptoms typically associated with VS-FCV, such as skin ulcers on the limbs, edema, or viremia, were observed. Notably, two of the hospitalized cats that showed severe disease had diarrhea and anemia, and died or had a prolonged illness. These cases reveal atypical symptoms of FCV infection that have not been previously reported. We further isolated typical strains from western Japan (Osaka, Kumamoto, and Ehime) and analyzed the viral genes along with virulent strains from Aoshima. Phylogenetic analysis showed that the Aoshima strain formed a new lineage distinct from known FCVs. The Aoshima strains isolated in the initial outbreak before December 5, 2020, and those isolated after the end of the outbreak, which are suspected pathogenic and typical non-pathogenic strains, respectively, were located in the same cluster and shown to be very similar in sequence. The virulent Aoshima strain, which causes atypical FCV infections in cats, may have been derived by acquiring several mutations from a typical strain that chronically infects cats on a remote island.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"353 ","pages":"Article 199535"},"PeriodicalIF":2.7000,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virus research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168170225000115","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In November 2020, a volunteer group reported an outbreak of an infectious disease with a high fatality rate and flu-like symptoms among stray cats in Aoshima, a remote island in Ehime, Japan. Nine adult cats with severe symptoms were hospitalized. Feline calicivirus (FCV) was isolated from pharyngeal swabs of six hospitalized cats. An outbreak of virulent systemic FCV (VS-FCV) infection was initially suspected because of obvious flu-like symptoms in adult cats; however, no symptoms typically associated with VS-FCV, such as skin ulcers on the limbs, edema, or viremia, were observed. Notably, two of the hospitalized cats that showed severe disease had diarrhea and anemia, and died or had a prolonged illness. These cases reveal atypical symptoms of FCV infection that have not been previously reported. We further isolated typical strains from western Japan (Osaka, Kumamoto, and Ehime) and analyzed the viral genes along with virulent strains from Aoshima. Phylogenetic analysis showed that the Aoshima strain formed a new lineage distinct from known FCVs. The Aoshima strains isolated in the initial outbreak before December 5, 2020, and those isolated after the end of the outbreak, which are suspected pathogenic and typical non-pathogenic strains, respectively, were located in the same cluster and shown to be very similar in sequence. The virulent Aoshima strain, which causes atypical FCV infections in cats, may have been derived by acquiring several mutations from a typical strain that chronically infects cats on a remote island.
在日本爱媛的一个偏远岛屿上,从几只表现出严重流感样症状的非典型感染的成年猫中分离出的猫杯状病毒的分子特征。
2020年11月,一个志愿者组织报告说,在日本爱媛偏远岛屿青山的流浪猫中爆发了一种高致死率和流感样症状的传染病。9只出现严重症状的成年猫被送入医院。从6只住院猫咽拭子中分离到猫杯状病毒(FCV)。由于在成年猫中出现明显的流感样症状,最初怀疑发生了致命性全身性FCV (VS-FCV)感染暴发;然而,没有观察到与vsfcv相关的典型症状,如四肢皮肤溃疡、水肿或病毒血症。值得注意的是,住院的猫中有两只出现严重疾病的猫患有腹泻和贫血,死亡或长期患病。这些病例显示了以前未报告的FCV感染的非典型症状。我们进一步从日本西部(大阪、熊本和爱媛)分离出典型菌株,并与来自广岛的毒力菌株一起分析病毒基因。系统发育分析表明,青岛株形成了一个不同于已知fcv的新谱系。2020年12月5日之前首次疫情分离的青山菌株和疫情结束后分离的疑似致病性菌株和典型非致病性菌株位于同一聚集性,序列非常相似。在猫中引起非典型FCV感染的致命的青山毒株,可能是从一个偏远岛屿上长期感染猫的典型毒株获得几个突变而来的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Virus research
Virus research 医学-病毒学
CiteScore
9.50
自引率
2.00%
发文量
239
审稿时长
43 days
期刊介绍: Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信