Yuntao Yan, Ya Chen, Xiaoya Zhu, Yan Wang, Hui Qi, Jinxin Gui, Haiqing Zhang, Jiwai He
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引用次数: 0
Abstract
The tiller angle, one of the critical factors that determine the rice plant type, is closely related to rice yield. An appropriate rice tiller angle can improve rice photosynthetic efficiency and increase yields. In this study, we identified a transcription factor, TILLRE ANGLE CONTROL 8 (TAC8), that is highly expressed in the rice tiller base and positively regulates the tiller angle by regulating cell length and endogenous auxin content; TAC8 encodes a TEOSINTE BRANCHED1/CYCLOIDEA/PCF transcriptional activator that is highly expressed in the nucleus. RNA-seq revealed that TAC8 is involved mainly in the photoperiod and abiotic stress response in rice. Yeast two-hybrid assays verified that TAC8 interacts with CHLOROPHYLL A/B-BINDING PROTEIN 1, which responds to photoperiod, and haplotype analysis revealed that a 34-bp deletion at position 1516 in the promoter region and a 9-bp deletion at position 153 in the coding region can result in impaired function or loss of function of TAC8. This study provides a new genetic resource for designing ideal plant types with appropriate rice tiller angle.
分蘖角度是决定水稻株型的关键因素之一,与水稻产量密切相关。适当的分蘖角度可以提高水稻的光合效率,提高产量。在本研究中,我们发现了一个在水稻分蘖基部高表达的转录因子——TILLRE ANGLE CONTROL 8 (TAC8),它通过调节细胞长度和内源生长素含量来正向调节分蘖角;TAC8编码一个在细胞核中高度表达的TEOSINTE BRANCHED1/CYCLOIDEA/PCF转录激活子。RNA-seq分析表明,TAC8主要参与水稻的光周期和非生物胁迫响应。酵母双杂交实验证实,TAC8与叶绿素A/B-BINDING PROTEIN 1相互作用,对光周期有响应。单倍型分析显示,启动子区1516位缺失34 bp,编码区153位缺失9 bp,可导致TAC8功能受损或功能丧失。本研究为设计水稻分蘖角度合适的理想株型提供了新的遗传资源。
期刊介绍:
Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.