Amino acid transporter OsATL13 coordinately regulates rice yield and quality by transporting phenylalanine and methionine

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-01-27 DOI:10.1016/j.plantsci.2025.112398

Lianxin Ding , Weiting Huang , Zhenghan Li, Zhongming Fang

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引用次数: 0

Abstract

Amino acids are crucial nutrients for growth in crops. In this study, we found an amino acid transporter-like 13 (OsATL13), that coordinately determined rice yield and quality. OsATL13 was primarily expressed in the root and panicle, its protein was localized on plasma membrane, and it principally transported phenylalanine and methionine. Overexpression (OE) of OsATL13 increased the tiller number by 31.4 %, resulting in a 16.18 % increase in grain yield compared to Zhonghua 11 (ZH11). It also decreased amylose content and increased protein content in OsATL13 OE lines compared to ZH11, whereas the OsATL13 mutant exhibited opposite effects. RNA-seq analysis revealed that upregulation of OsATL13 influenced the expression of genes associated with nitrogen and starch metabolism pathways. Notably, exogenous treatment with phenylalanine and methionine promoted axillary buds outgrowth, increased tiller number and rice yield, improved milled and head rice rates, and decreased chalky rice rate. Furthermore, rapid viscosity analysis supported the observation that phenylalanine and methionine treatments influenced rice eating and cooking quality. This research offers new perspectives on the synchronized enhancement of both rice yield and quality with amino acid transporter OsATL13.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.