Maslinic acid improves mitochondrial function and inhibits oxidative stress and autophagy in human gastric smooth muscle cells.

IF 1.7 4区 生物学 Q3 BIOLOGY
Open Life Sciences Pub Date : 2025-01-27 eCollection Date: 2025-01-01 DOI:10.1515/biol-2022-1036
Xiaoying Zheng, Shuning Zhang, Qiaobin Chen
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引用次数: 0

Abstract

Functional dyspepsia (FD) is a chronic disease that occurs in the gastroduodenal region and significantly impacts human health. Maslinic acid (MA), a pentacyclic triterpene acid, is the primary bioactive ingredient in Chinese medicinal herbs such as hawthorn, which exhibits beneficial impacts on the regulation of various disease progressions. However, the specific functions and associated pathways of MA in FD progression remain unclear and require further investigation. In this work, it was demonstrated that MA enhanced the cell viability of human gastric smooth muscle cells (HGSMCs). In addition, the mitochondrial dysfunctions induced by carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were rescued after MA treatment. Furthermore, autophagy was increased following CCCP treatment, but this phenomenon was counteracted after MA treatment. The oxidative stress, elevated after CCCP treatment, was alleviated following MA addition. Finally, the AMPK/SIRT1 pathway was suppressed after CCCP stimulation but was re-activated after MA treatment. In conclusion, it was uncovered that MA accelerated HGSMC viability and improved mitochondrial function, inhibited autophagy, alleviated oxidative stress, and stimulated the AMPK/SIRT1 pathway. This discovery may offer new insight into the therapeutic effects of MA in FD progression.

马山酸改善人胃平滑肌细胞线粒体功能,抑制氧化应激和自噬。
功能性消化不良(FD)是一种发生在胃十二指肠区的慢性疾病,严重影响人体健康。山楂酸(Maslinic acid, MA)是一种五环三萜酸,是山楂等中草药的主要生物活性成分,对调节多种疾病进展具有有益作用。然而,MA在FD进展中的具体功能和相关途径尚不清楚,需要进一步研究。本研究证实,MA可增强人胃平滑肌细胞(HGSMCs)的细胞活力。此外,3-氯苯基腙(CCCP)诱导的线粒体功能障碍在MA处理后得到恢复。此外,CCCP处理后自噬增加,但MA处理后这种现象被抵消。氧化应激在CCCP处理后升高,在添加MA后得到缓解。最后,AMPK/SIRT1通路在CCCP刺激后被抑制,但在MA处理后被重新激活。综上所述,我们发现MA可以加速HGSMC的活力,改善线粒体功能,抑制自噬,减轻氧化应激,刺激AMPK/SIRT1通路。这一发现可能为MA在FD进展中的治疗作用提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.50
自引率
4.50%
发文量
131
审稿时长
43 weeks
期刊介绍: Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
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