Global identification and regulatory network analysis reveal the significant roles of lncRNAs during anther and pollen development in Arabidopsis.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Dong Zhou, Ruiqi Song, Yuan Fang, Rui Liu, Chenjiang You, Yijie Wang, Li Huang
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引用次数: 0

Abstract

Key message: A high-throughput sequencing identified 1283 lncRNAs in anthers at different stages in Arabidopsis and their relationship with protein-coding genes and miRNAs during anther and pollen development were analyzed. Long non-coding RNAs (lncRNAs) are important regulatory molecules involved in various biological processes. However, their roles in male reproductive development and interactions with miRNAs remained elusive. In this study, a high-throughput sequencing of anthers at different developmental stages in Arabidopsis identified 1283 lncRNAs including 524 differentially expressed lncRNAs (DELs). Most of these DELs exhibited positive correlations with the expression patterns of adjacent protein-coding genes. Weighted gene co-expression network analysis (WGCNA) revealed that protein-coding genes targeted by DELs in four modules related to the tetrad stage were associated with functions such as pollen wall formation, pollen germination, or pollen tube growth, respectively. Furthermore, five, 10, and 11 lncRNAs were predicted as miRNAs' endogenous target mimics (eTMs), precursors, and natural antisense transcripts of pri-miRNA, respectively. Remarkably, the lncRNA, host gene of ath-miR167a (ath-miR167aHG), predicted as the precursor of miR167a, was selected for function validation. Its overexpression resulted in the up-regulation of miR167a and the subsequent down-regulation of miR167a's target genes ARF6 and ARF8, demonstrating a functional interaction between ath-miR167aHG and miR167a. The transgenic plants showed delayed flowering, shorter filaments, abnormal anther dehiscence, and undeveloped siliques ultimately, suggesting a role of ath-miR167aHG in male reproductive development. Collectively, our research shed new light on the functions of lncRNAs in male reproductive development and uncovered the unique interactions between lncRNAs and miRNAs.

全球鉴定和调控网络分析揭示了lncrna在拟南芥花药和花粉发育中的重要作用。
通过高通量测序,鉴定了拟南芥花药不同发育阶段的1283个lncrna,并分析了它们与花药和花粉发育过程中蛋白编码基因和mirna的关系。长链非编码rna (lncRNAs)是参与多种生物过程的重要调控分子。然而,它们在男性生殖发育中的作用以及与mirna的相互作用仍然难以捉摸。本研究通过对拟南芥不同发育阶段花药的高通量测序,鉴定出1283个lncrna,其中差异表达lncrna (DELs) 524个。大多数DELs与邻近蛋白编码基因的表达模式呈正相关。加权基因共表达网络分析(Weighted gene co-expression network analysis, WGCNA)显示,DELs在四分体期相关的四个模块中靶向的蛋白编码基因分别与花粉壁形成、花粉萌发或花粉管生长等功能相关。此外,有5种、10种和11种lncrna分别被预测为mirna的内源性靶模拟物(eTMs)、前体和pri-miRNA的天然反义转录物。值得注意的是,被预测为miR167a前体的ath-miR167a宿主基因lncRNA (ath-miR167aHG)被选中进行功能验证。其过表达导致miR167a上调,随后miR167a的靶基因ARF6和ARF8下调,表明ath-miR167aHG与miR167a之间存在功能相互作用。转基因植株开花延迟、花丝缩短、花药开裂异常、角蕊发育不全,表明ath-miR167aHG在雄性生殖发育中起作用。总的来说,我们的研究揭示了lncRNAs在男性生殖发育中的功能,揭示了lncRNAs和miRNAs之间独特的相互作用。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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