Responses of skeletal muscle to mechanical stimuli in female rats following and during muscle disuse atrophy.

Abstract

The purpose of this study was to investigate the ability of mechanotherapy to enhance recovery or prevent loss of muscle size with disuse in female rats. Female F344/BN rats were assigned to weight bearing (WB), hindlimb suspended (HS) for 14 days with reambulation for 7 days without mechanotherapy or reambulation (RA) with mechanotherapy (RAM) (study 1), or to WB, HS for 7 days, with HS mechanotherapy (HSM) or without mechanotherapy (study 2) to gastrocnemius muscle. Muscle fiber cross-sectional area (CSA) and fiber type, collagen, satellite cell number, and protein synthesis (k_syn) and degradation (k_deg) were assessed. Study 1: muscle weight, but not CSA, was higher in RAM compared with HS, but CSA was higher in RA compared with HS. Myofibrillar k_syn was higher in RA and RAM compared to WB and HS but not different between RA and RAM. Myofibrillar k_deg was lower with mechanotherapy compared to HS. Study 2: muscle weight, CSA, and myofibrillar k_syn and k_deg were not different with mechanotherapy. Collagen content was lower with mechanotherapy but collagen k_syn was not. Mechanotherapy was not associated with changes in fiber type, satellite cell, or myonuclear number in either study. Compared to males, female rats had less muscle loss with HS, which was associated with less loss of myofibrillar k_syn. Recovery from atrophy was associated with higher k_syn in female and lower k_deg in male rats. Female rat muscles do not exhibit a growth response to mechanotherapy with disuse or reambulation. Furthermore, male and female rats show distinct responses to different mechanical stimuli.NEW & NOTEWORTHY This study investigates the response of female rats to mechanical stimulation in both active and passive forms following and during muscle disuse atrophy. New findings indicate that female rats respond to active loading with enhanced muscle regrowth and protein synthesis, whereas passive loading using mechanotherapy did not affect atrophy or recovery of female muscles. Comparison with published data indicates that there are distinct differences in male and female rats in their response to mechanical stimuli.