First genome sequence of a purple sulphur bacterium of the genus Thioalkalicoccus, its characterization as a new isolate of Thioalkalicoccus limnaeus and proposal of strain Um2 as neotype of this species.

IF 2 3区 生物学 Q4 MICROBIOLOGY
Irina A Bryantseva, John A Kyndt, Johannes F Imhoff
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引用次数: 0

Abstract

A new alkaliphilic strain of a purple sulphur bacterium designated as Um2 (=KCTC 25734=VKM B-3893=UQM 41073) with bacteriochlorophyll b and internal photosynthetic membranes of tubular type was isolated from the Umhei hydrothermal system (40 °C, pH 9.3 and salinity 0.42 g l-1) located in the Baikal rift zone (Russia). Based on morphological and physiological characteristics, this bacterium was classified as Thioalkalicoccus limnaeus. The 16S rRNA gene sequence similarity of strain Um2 was 96.69% with the type strain of Tac. limnaeus A26T, 95.41% with 'Thioflavicoccus mobilis' 8321T and 95.34% with Thiococcus pfennigii 4250. The level of similarity of the ribulose 1,5-bisphosphate carboxylase sequences of strain Um2 and known strains of Thioalkalicoccus showed that they belong to the same species. Comparison of the genome nt sequences of strain Um2 revealed that the new isolate was remote from all other described Chromatiaceae species both in digital DNA-DNA hybridization (21.5%) and in average nt identity (76.7%) at the genus level. However, a genome nt sequence had not been determined for any of the known Thioalkalicoccus strains; therefore, the first genome sequence of a member of the genus Thioalkalicoccus is presented here. Tac. limnaeus Um2 is proposed as the neotype, as strain A26T has been lost from culture collections.

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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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